A simple and fast method for quantification of ertapenem using meropenem as internal standard in human plasma in a clinical setting

被引：10

作者：

D'Avolio, Antonio ^{[1
]}

Baietto, Lorena ^{[1
]}

De Rosa, Francesco Giuseppe ^{[1
]}

Garazzino, Silvia ^{[1
]}

Sciandra, Mauro ^{[1
]}

Siccardi, Marco ^{[1
]}

Bonora, Stefano ^{[1
]}

Di Perri, Giovanni ^{[1
]}

机构：

[1] Univ Turin, Amedeo Savoia Hosp, Dept Infect Dis, Turin, Italy

来源：

THERAPEUTIC DRUG MONITORING | 2008年 / 30卷 / 01期

关键词：

ertapenem; HPLC-UV; human plasma; meropenem; TDM;

D O I：

10.1097/FTD.0b013e31815f4f7e

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 [基础医学];

摘要：

A high-performance liquid chromatography method for the determination of ertapenem in human plasma using ultraviolet detection (300 nm) was developed and validated. The method involved a simple organic protein precipitation that guarantees rapid sample preparation. By using meropenem as internal standard, it also guarantees improved precision (relative standard deviation <3.70) and accuracy (deviation from true Value <6.58%). The method was linear from 0.59 to 150 mu g/mL and reproducible. In conclusion, this method allows the measurement of ertapenem in human plasma and may be used for both clinical routine applications and pharmacokinetic studies.

引用

页码：90 / 94

页数：5

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