Tryptase activates peripheral blood mononuclear cells causing the synthesis and release of TNF-α, IL-6 and IL-1β:: possible relevance to multiple sclerosis

被引：56

作者：

Malamud, V

Vaaknin, A

Abramsky, O

Mor, M

Burgess, LE

Ben-Yehudah, A

Lorberboum-Galski, H

机构：

[1] Hebrew Univ Jerusalem, Hadassah Med Sch, Dept Cellular Biochem & Human Genet, IL-91120 Jerusalem, Israel

[2] Hebrew Univ Jerusalem, Hadassah Hosp, Dept Neurol, IL-91120 Jerusalem, Israel

[3] ARRAY BioPharma, Boulder, CO 80301 USA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2003年 / 138卷 / 1-2期

关键词：

tryptase; peripheral blood mononuclear cells; multiple sclerosis; cytokines;

D O I：

10.1016/S0165-5728(03)00090-0

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Presence of mast cells and an increase in the concentration of their products has been reported in multiple sclerosis (MS) plaques. The most abundant secretory mediator of the human mast cell is the tetrameric protease tryptase. We demonstrate that tryptase can activate peripheral mononuclear cells (PBMCs), isolated from healthy donors as well as MS patients for the release of tumor necrosis factor-a (TNF-alpha), interleukin (IL)-6 and IL-1beta. Cytokine secretion was significantly higher in secondary progressive (SP) MS patients and healthy control (HC) individuals than in relapsing-remitting (RR) patients. Our findings suggest that tryptase is, most probably, an important mediator of inflammation in MS. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：115 / 122

页数：8

共 46 条

[1] Activation of protease-activated receptor (PAR)-1, PAR-2, and PAR-4 stimulates IL-6, IL-8, and prostaglandin E2 release from human respiratory epithelial cells [J].