Phase-fluorimetry study on dielectric relaxation of human serum albumin

被引：13

作者：

Buzády, A

Erostyák, J

Somogyi, B

机构：

[1] Univ Pecs, Inst Phys, Dept Expt Phys, H-7624 Pecs, Hungary

[2] Univ Pecs, Dept Biophys, H-7625 Pecs, Hungary

[3] Univ Pecs, Jobin Yvon Mol Luminescence Reference Lab, H-7624 Pecs, Hungary

[4] Hungarian Acad Sci, Dept Biophys, Res Grp, H-7625 Pecs, Hungary

来源：

BIOPHYSICAL CHEMISTRY | 2000年 / 88卷 / 1-3期

关键词：

dielectric relaxation; human serum albumin; phase-fluorometry; time-emission matrix;

D O I：

10.1016/S0301-4622(00)00210-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The dielectric relaxation (DR) of human serum albumin (HSA) was studied by the method of phase-fluorometry, The protein environment of the single tryptophan in HSA shows a relatively low-speed DR of sub-ns characteristic time. This relaxation can be measured as a decaying red-shift of the time-resolved fluorescence emission spectra. The details of calculations of time-emission matrices (TEM) and comparison to the fluorescence data of the reference solution of N-acetyl-L-tryptophanamide (NATA) are also presented. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：153 / 163

页数：11

共 35 条

[1] INTERPRETATION OF FLUORESCENCE DECAYS IN PROTEINS USING CONTINUOUS LIFETIME DISTRIBUTIONS [J].