Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni

被引：386

作者：

Verjovski-Almeida, S ^{[1
]}

DeMarco, R

Martins, EAL

Guimaraes, PEM

Ojopi, EPB

Paquola, ACM

Piazza, JP

Nishiyama, MY

Kitajima, JP

Adamson, RE

Ashton, PD

Bonaldo, MF

Coulson, PS

Dillon, GP

Farias, LP

Gregorio, SP

Ho, PL

Leite, RA

Malaquias, LCC

Marques, RCP

Miyasato, PA

Nascimento, ALTO

Ohlweiler, FP

Reis, EM

Ribeiro, MA

Sá, RG

Stukart, GC

Soares, MB

Gargioni, C

Kawano, T

Rodrigues, V

Madeira, AMBN

Wilson, RA

Menck, CFM

Setubal, JC

Leite, LCC

Dias-Neto, E

机构：

[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508900 Sao Paulo, Brazil

[2] Inst Butantan, Ctr Biotecnol, BR-05503900 Sao Paulo, Brazil

[3] Univ Sao Paulo, HCFM, Inst Psiquiatria, Lab Neurosci LIM27, BR-05403010 Sao Paulo, Brazil

[4] Univ Sao Paulo, Inst Quim, Dept Bioquim, Lab Bioinformat, BR-05508900 Sao Paulo, Brazil

[5] Univ Estadual Campinas, Inst Computacao, BR-13084971 Campinas, SP, Brazil

[6] Univ York, Dept Biol, York YO10 5YW, N Yorkshire, England

[7] Univ Iowa, Dept Pediat, Iowa City, IA 52242 USA

[8] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo, Brazil

[9] Univ Vale Rio Doce, BR-35030390 Governador Valadares, MG, Brazil

[10] Inst Butantan, Parasitol Lab, BR-05503900 Sao Paulo, Brazil

[11] Univ Sao Paulo, Fac Med Vet & Zootecnia, BR-05508900 Sao Paulo, Brazil

[12] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, BR-14049900 Ribeirao Preto, SP, Brazil

[13] Univ Iowa, Dept Biochem, Iowa City, IA 52242 USA

[14] Univ Iowa, Dept Orthopaed, Iowa City, IA 52242 USA

[15] Univ Iowa, Dept Physiol, Iowa City, IA 52242 USA

[16] Univ Iowa, Dept Biophys, Iowa City, IA 52242 USA

[17] Inst Adolfo Lutz Registro, Dept Parasitol, BR-01246902 Sao Paulo, Brazil

来源：

NATURE GENETICS | 2003年 / 35卷 / 02期

基金：

巴西圣保罗研究基金会; 英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1038/ng1237

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Schistosoma mansoni is the primary causative agent of schistosomiasis, which affects 200 million individuals in 74 countries. We generated 163,000 expressed-sequence tags ( ESTs) from normalized cDNA libraries from six selected developmental stages of the parasite, resulting in 31,000 assembled sequences and 92% sampling of an estimated 14,000 gene complement. By analyzing automated Gene Ontology assignments, we provide a detailed view of important S. mansoni biological systems, including characterization of metazoa-specific and eukarya-conserved genes. Phylogenetic analysis suggests an early divergence from other metazoa. The data set provides insights into the molecular mechanisms of tissue organization, development, signaling, sexual dimorphism, host interactions and immune evasion and identifies novel proteins to be investigated as vaccine candidates and potential drug targets.

引用

页码：148 / 157

页数：10

共 51 条

[1] The genome sequence of Drosophila melanogaster [J].

Adams, MD ;

Celniker, SE ;

Holt, RA ;

Evans, CA ;

Gocayne, JD ;

Amanatides, PG ;

Scherer, SE ;

Li, PW ;

Hoskins, RA ;

Galle, RF ;

George, RA ;

Lewis, SE ;

Richards, S ;

Ashburner, M ;

Henderson, SN ;

Sutton, GG ;

Wortman, JR ;

Yandell, MD ;

Zhang, Q ;

Chen, LX ;

Brandon, RC ;

Rogers, YHC ;

Blazej, RG ;

Champe, M ;

Pfeiffer, BD ;

Wan, KH ;

Doyle, C ;

Baxter, EG ;

Helt, G ;

Nelson, CR ;

Miklos, GLG ;

Abril, JF ;

Agbayani, A ;

An, HJ ;

Andrews-Pfannkoch, C ;

Baldwin, D ;

Ballew, RM ;

Basu, A ;

Baxendale, J ;

Bayraktaroglu, L ;

Beasley, EM ;

Beeson, KY ;

Benos, PV ;

Berman, BP ;

Bhandari, D ;

Bolshakov, S ;

Borkova, D ;

Botchan, MR ;

Bouck, J ;

Brokstein, P .

SCIENCE, 2000, 287 (5461) :2185-2195

[2] SCHISTOSOMA-MANSONI - NUCLEIC-ACID SYNTHESIS IN IMMATURE FEMALES FROM SINGLE-SEX INFECTIONS, PAIRED INVITRO WITH INTACT MALES AND MALE SEGMENTS [J].

BASCH, PF .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1988, 90 (02) :389-392

[3] Using RNA interference to manipulate endogenous gene expression in Schistosoma mansoni sporocysts [J].

Boyle, JP ;

Wu, XJ ;

Shoemaker, CB ;

Yoshino, TP .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2003, 128 (02) :205-215

[4] Genome sequence of the nematode C-elegans:: A platform for investigating biology [J].

不详 .

SCIENCE, 1998, 282 (5396) :2012-2018

[5] The radiation-attenuated vaccine against schistosomes in animal models: Paradigm for a human vaccine? [J].

Coulson, PS .

ADVANCES IN PARASITOLOGY, VOL 39, 1997, 39 :271-336

[6] Elimination of a primary schistosome infection from rats coincides with elevated IgE titres and mast cell degranulation [J].

Cutts, L ;

Wilson, RA .

PARASITE IMMUNOLOGY, 1997, 19 (02) :91-102

[7] ALLERGENS OF SCHISTOSOMA-MANSONI .2. FRACTIONATION AND CHARACTERIZATION OF S-MANSONI EGG ALLERGENS [J].

DAMONNEVILLE, M ;

PIERCE, RJ ;

VERWAERDE, C ;

CAPRON, A .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1984, 73 (03) :248-255

[8] Hormones and nuclear receptors in schistosome development [J].

de Mendonça, RL ;

Escrivá, H ;

Bouton, D ;

Laudet, V ;

Pierce, RJ .

PARASITOLOGY TODAY, 2000, 16 (06) :233-240

[9] The draft genome of Ciona intestinalis:: Insights into chordate and vertebrate origins [J].

Dehal, P ;

Satou, Y ;

Campbell, RK ;

Chapman, J ;

Degnan, B ;

De Tomaso, A ;

Davidson, B ;

Di Gregorio, A ;

Gelpke, M ;

Goodstein, DM ;

Harafuji, N ;

Hastings, KEM ;

Ho, I ;

Hotta, K ;

Huang, W ;

Kawashima, T ;

Lemaire, P ;

Martinez, D ;

Meinertzhagen, IA ;

Necula, S ;

Nonaka, M ;

Putnam, N ;

Rash, S ;

Saiga, H ;

Satake, M ;

Terry, A ;

Yamada, L ;

Wang, HG ;

Awazu, S ;

Azumi, K ;

Boore, J ;

Branno, M ;

Chin-bow, S ;

DeSantis, R ;

Doyle, S ;

Francino, P ;

Keys, DN ;

Haga, S ;

Hayashi, H ;

Hino, K ;

Imai, KS ;

Inaba, K ;

Kano, S ;

Kobayashi, K ;

Kobayashi, M ;

Lee, BI ;

Makabe, KW ;

Manohar, C ;

Matassi, G ;

Medina, M .

SCIENCE, 2002, 298 (5601) :2157-2167

[10] Molecular characterization and immunolocalization of Schistosoma mansoni ATP-diphosphohydrolase [J].

DeMarco, R ;

Kowaltowski, AT ;

Mortara, RA ;

Verjovski-Almeida, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 307 (04) :831-838

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