Molecular characterization of the gene encoding the DNA gyrase a subunit of Streptococcus pneumoniae

被引:40
作者
Balas, D [1 ]
Fernández-Moreira, E [1 ]
De la Campa, AG [1 ]
机构
[1] Inst Salud Carlos III, Unidad Genet Bacteriana, Consejo Super Invest Cient, Ctr Nacl Biol Fundamental, Madrid 28220, Spain
关键词
D O I
10.1128/JB.180.11.2854-2861.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The gene encoding the DNA gyrase A subunit of Streptococcus pneumoniae was cloned and sequenced. The gyrA gene codes for a protein of 822 amino acids homologous to the gyrase A subunit of eubacteria. Translation of the gene in an Escherichia coli expression system revealed a 92-kDa polypeptide. A sequence directed DNA curvature was identified in the promoter region of gyrA. The bend center was mapped and located between the -35 and -10 regions of the promoter. Primer extension analysis showed that gyrA transcription initiates 6 bp downstream of an extended -10 promoter. The possible implications of the bent DNA region as a regulatory element in the transcription of gyrA are discussed.
引用
收藏
页码:2854 / 2861
页数:8
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