Molecular characterization of the gene encoding the DNA gyrase a subunit of Streptococcus pneumoniae
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Balas, D
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Inst Salud Carlos III, Unidad Genet Bacteriana, Consejo Super Invest Cient, Ctr Nacl Biol Fundamental, Madrid 28220, SpainInst Salud Carlos III, Unidad Genet Bacteriana, Consejo Super Invest Cient, Ctr Nacl Biol Fundamental, Madrid 28220, Spain
Balas, D
[1
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Fernández-Moreira, E
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Inst Salud Carlos III, Unidad Genet Bacteriana, Consejo Super Invest Cient, Ctr Nacl Biol Fundamental, Madrid 28220, SpainInst Salud Carlos III, Unidad Genet Bacteriana, Consejo Super Invest Cient, Ctr Nacl Biol Fundamental, Madrid 28220, Spain
Fernández-Moreira, E
[1
]
De la Campa, AG
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Inst Salud Carlos III, Unidad Genet Bacteriana, Consejo Super Invest Cient, Ctr Nacl Biol Fundamental, Madrid 28220, SpainInst Salud Carlos III, Unidad Genet Bacteriana, Consejo Super Invest Cient, Ctr Nacl Biol Fundamental, Madrid 28220, Spain
De la Campa, AG
[1
]
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[1] Inst Salud Carlos III, Unidad Genet Bacteriana, Consejo Super Invest Cient, Ctr Nacl Biol Fundamental, Madrid 28220, Spain
The gene encoding the DNA gyrase A subunit of Streptococcus pneumoniae was cloned and sequenced. The gyrA gene codes for a protein of 822 amino acids homologous to the gyrase A subunit of eubacteria. Translation of the gene in an Escherichia coli expression system revealed a 92-kDa polypeptide. A sequence directed DNA curvature was identified in the promoter region of gyrA. The bend center was mapped and located between the -35 and -10 regions of the promoter. Primer extension analysis showed that gyrA transcription initiates 6 bp downstream of an extended -10 promoter. The possible implications of the bent DNA region as a regulatory element in the transcription of gyrA are discussed.