Microarray-based genotyping for blood groups:: comparison of gene array and 5′-nuclease assay techniques with human platelet antigen as a model

被引：51

作者：

Bugert, P

McBride, S

Smith, G

Dugrillon, A

Klüter, H

Ouwehand, WH

Metcalfe, P

机构：

[1] Natl Inst Biol Stand & Controls, Potters Bar EN6 3QG, Herts, England

[2] Univ Heidelberg, Inst Transfus Med & Immunol, Fac Clin Med, Mannheim, Germany

[3] Univ Cambridge, Dept Haematol, Cambridge, England

[4] Natl Blood Serv, Cambridge, England

来源：

TRANSFUSION | 2005年 / 45卷 / 05期

关键词：

D O I：

10.1111/j.1537-2995.2005.04318.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

BACKGROUND: Most blood group alloantigens specific for red cells and platelets (PLTs) are based on single-nucleotide polymorphisms (SNPs) in genes encoding relevant membrane proteins. STUDY DESIGN AND METHODS: By use of five human PLT antigen (HPA) systems as a model, the suitability of a fourth-generation microarray technique for SNP typing was investigated. The results of the former were compared with those of a parallel developed third-generation technique (TaqMan assay, Applied Biosystems). Both techniques were validated by use of a unique panel of 71 blinded DNA samples containing at least 15 aa, bb, and ab genotypes for the HPA-1, -2, -3, -5, and-15 systems. RESULTS: Unambiguous and concordant results were obtained with both techniques for all samples. CONCLUSION: The data presented here validate the use of microarray for large-scale SNP typing for clinically relevant blood group alloantigens.

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页码：654 / 659

页数：6

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