Protective effect of L-carnitine on renal ischaemia-reperfusion injury in the rat

This study was designed to investigate the effect of L-carnitine in ischaemia and reperfusion of the rat kidney. Rats were randomly allocated into three groups. Group I (control group; n = 6) received no treatment. Group II (isotonic saline group; n = 6), received 2 ml of isotonic saline 15 min before the renal ischaemia, and group III (carnitine group; n = 6) received L-carnitine hydrochloride (100 mg kg(-1)) intraperitoneally. At the end of the reperfusion period, rats were sacrificed. Tissue malondialdehyde level (MDA), myeloperoxidase (MPO) activity, and nitrite/nitrate (NO) level of renal tissue were measured to evaluate the lipid peroxidation, neutrophil function, and nitric oxide metabolism, respectively. The tissue levels of MDA, MPO and NO were lower in group III (71.8 +/- 8.4, 172.1 +/- 27.4 Ug(-1) tissue, 76.3 +/- 29.7 mu mol l(-1) respectively) than levels in groups I (103.4 +/- 13.4 nmolg(-1), 325.9 +/- 20.2 Ug(-1) tissue, 144.5 +/- 39.2 mu mol l(-1), respectively) and II (103.5 +/- 11.4 nmol g(-1),317.1 +/- 41.5 Ug(-1) tissue, 148.9 +/- 23.9 mu mol l(-1), respectively). It is shown that carnitine protects kidney tissue against ischaemia-reperfusion injury. Copyright (c) 2004 John Wiley & Sons, Ltd.