Regeneration of pulpo-dentinal-like complex by a group of unique multipotent CD24a+ stem cells

被引：66

作者：

Chen, Hong ^{[1
,2
,3
,4
]}

Fu, Huancheng ^{[1
]}

Wu, Xue ^{[1
]}

Duan, Yufeng ^{[1
,2
,3
,4
]}

Zhang, Sicheng ^{[1
,2
,3
,4
]}

Hu, Hong ^{[1
]}

Liao, Yuansong ^{[1
]}

Wang, Tao ^{[1
,2
,3
,4
]}

Yang, Yan ^{[1
,2
,3
,4
]}

Chen, Guoqing ^{[2
,5
]}

Li, Zhonghan ^{[1
,2
]}

Tian, Weidong ^{[1
,2
,3
,4
]}

机构：

[1] Sichuan Univ, Key Lab Bioresource & Ecoenvironm, Minist Educ,West China Hosp Stomatol,Coll Life Sc, Ctr Growth Metab & Aging,Coll Life Sci,State Key, Chengdu, Peoples R China

[2] Sichuan Univ, West China Hosp Stomatol, Natl Engn Lab Oral Regenerat Med, Chengdu, Peoples R China

[3] Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Chengdu, Peoples R China

[4] Sichuan Univ, West China Hosp Stomatol, Dept Oral & Maxillofacial Surg, Chengdu, Peoples R China

[5] Univ Elect Sci & Technol China, Sch Med, Chengdu, Peoples R China

来源：

SCIENCE ADVANCES | 2020年 / 6卷 / 15期

基金：

中国国家自然科学基金;

关键词：

HUMAN DENTAL-PULP; HUMAN MUSE CELLS; APICAL PAPILLA; SELF-RENEWAL; DIFFERENTIATION; FOLLICLE; LGR6;

D O I：

10.1126/sciadv.aay1514

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Dental pulp is critical to maintain the vitality of a tooth. Regeneration of pulpo-dentinal complex is of great interest to treat pulpitis and pulp necrosis. In this study, through three-dimensional spheroid culture, a group of unique multipotent stem cells were identified from mouse dental papilla called multipotent dental pulp regenerative stem cells (MDPSCs). MDPSCs exhibited enhanced osteogenic/odontogenic differentiation capabilities and could form regenerative dentin and neurovascular-like structures that mimicked the native teeth in vivo. Further analysis revealed that CD24a was the bona fide marker for MDPSCs, and their expansion was highly dependent on the expression of a key transcriptional factor, Sp7 . Last, CD24a(+) cells could be detected in primary dental papilla in mice and human, suggesting that MDPSCs resided in their native niches. Together, our study has identified a previously unidentified group of multipotent pulp regenerative stem cells with defined molecular markers for the potential treatment of pulpitis and pulp necrosis.

引用

页数：14

共 40 条

[1]

Specificity Protein 7 Is Required for Proliferation and Differentiation of Ameloblasts and Odontoblasts [J].