A cellular system to study the role of nitric oxide in cell death, survival, and migration

被引：6

作者：

Bulotta, S

Perrotta, C

Cerullo, A

De Palma, C

Clementi, E

Borgese, N

机构：

[1] Univ Catanzaro Magna Graecia, Dept Pharmacobiol Sci, I-88100 Catanzaro, Italy

[2] Univ Calabria, Dept Pharmacobiol, I-87036 Arcavacata Di Rende, Italy

[3] Ist Sci San Raffaele, DIBIT H, I-20132 Milan, Italy

[4] CNR, Inst Neurosci Cell Mol Pharmacol, I-20133 Milan, Italy

来源：

NEUROTOXICOLOGY | 2005年 / 26卷 / 05期

关键词：

apoptosis; ceramide; endothelial NO synthase; PI3; kinase/Akt; TetOff system;

D O I：

10.1016/j.neuro.2005.01.011

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The gaseous messenger nitric oxide (NO) plays a bewildering number of roles in fundamental processes, such as cell locomotion, differentiation, proliferation and death. Its different and often contrasting roles may depend on its concentration and intracellular site of generation. We describe here a simple system with which to investigate the roles of NO generated at physiological levels in HeLa cells by eNOS transfected under an inducible promoter This system has allowed us to uncover unexpected signalling circuits between NO and ceramide, involved in the response of cells to apoptotic stimuli. At present, we are using these cells as a tool to investigate the role of NO in migration. (c) 2005 Elsevier Inc. All rights reserved.

引用

收藏

页码：841 / 845

页数：5

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