Development of a 5′ nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus

被引：122

作者：

Yoshida, A ^{[1
]}

Suzuki, N ^{[1
]}

Nakano, Y ^{[1
]}

Kawada, M ^{[1
]}

Oho, T ^{[1
]}

Koga, T ^{[1
]}

机构：

[1] Kyushu Univ, Fac Dent Sci, Dept Prevent Dent, Higashi Ku, Fukuoka 8128582, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 09期

关键词：

D O I：

10.1128/JCM.41.9.4438-4441.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 5' nuclease TaqMan PCR assay was developed for the quantitative detection of the major cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. The absolute and relative numbers of bacteria were measured by this method. This assay will be useful for quantifying these organisms in oral specimens and for analyzing biofilm formation.

引用

页码：4438 / 4441

页数：4

共 21 条

[1] MICROBIAL VARIATIONS IN APPROXIMAL DENTAL PLAQUE [J].

BOWDEN, GH ;

HARDIE, JM ;

SLACK, GL .

CARIES RESEARCH, 1975, 9 (04) :253-277

[2] OLIGONUCLEOTIDE PROBES FOR MUTANS STREPTOCOCCI [J].

CANGELOSI, GA ;

IVERSEN, JM ;

ZUO, Y ;

OSWALD, TK ;

LAMONT, RJ .

MOLECULAR AND CELLULAR PROBES, 1994, 8 (01) :73-80

[3] ENUMERATION OF MUTANS STREPTOCOCCI IN CLINICAL-SAMPLES BY USING MONOCLONAL-ANTIBODIES [J].

DESOET, JJ ;

VANDALEN, PJ ;

PAVICIC, MJAMP ;

DEGRAAFF, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (11) :2467-2472

[4] PCR PRIMERS AND PROBES FOR THE 16S RIBOSOMAL-RNA GENE OF MOST SPECIES OF PATHOGENIC BACTERIA, INCLUDING BACTERIA FOUND IN CEREBROSPINAL-FLUID [J].

GREISEN, K ;

LOEFFELHOLZ, M ;

PUROHIT, A ;

LEONG, D .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (02) :335-351

[5] CLONING OF A STREPTOCOCCUS-SOBRINUS-GTF GENE THAT ENCODES A GLUCOSYLTRANSFERASE WHICH PRODUCES A HIGH-MOLECULAR-WEIGHT WATER-SOLUBLE GLUCAN [J].

HANADA, N ;

YAMASHITA, Y ;

SHIBATA, Y ;

SATO, S ;

KATAYAMA, T ;

TAKEHARA, T ;

INOUE, M .

INFECTION AND IMMUNITY, 1991, 59 (10) :3434-3438

[6] Real time quantitative PCR [J].

Heid, CA ;

Stevens, J ;

Livak, KJ ;

Williams, PM .

GENOME RESEARCH, 1996, 6 (10) :986-994

[7]

Herzberg M C, 1997, Adv Dent Res, V11, P69

[8] DETECTION OF SPECIFIC POLYMERASE CHAIN-REACTION PRODUCT BY UTILIZING THE 5'-]3' EXONUCLEASE ACTIVITY OF THERMUS-AQUATICUS DNA-POLYMERASE [J].

HOLLAND, PM ;

ABRAMSON, RD ;

WATSON, R ;

GELFAND, DH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) :7276-7280

[9] A NEW METHOD FOR THE ESTIMATION OF MUTANS STREPTOCOCCI IN HUMAN-SALIVA [J].

JENSEN, B ;

BRATTHALL, D .

JOURNAL OF DENTAL RESEARCH, 1989, 68 (03) :468-471

[10] Immunization against dental caries [J].

Koga, T ;

Oho, T ;

Shimazaki, Y ;

Nakano, Y .

VACCINE, 2002, 20 (16) :2027-2044

← 1 2 3 →