Impact of Local versus Global Ligand Density on Cellular Adhesion

被引：139

作者：

Deeg, Janosch A. ^{[1
,2
]}

Louban, Ilia ^{[1
,2
]}

Aydin, Daniel ^{[1
,2
]}

Selhuber-Unkel, Christine ^{[3
]}

Kessler, Horst ^{[4
]}

Spatz, Joachim P. ^{[1
,2
]}

机构：

[1] Max Planck Inst Intelligent Syst, Dept New Mat & Biosyst, D-70569 Stuttgart, Germany

[2] Heidelberg Univ, Dept Biophys Chem, D-6900 Heidelberg, Germany

[3] Univ Kiel, Inst Zool, D-24098 Kiel, Germany

[4] Tech Univ Munich, Inst Organ Chem & Biochem, Lehrstuhl 2, D-85747 Garching, Germany

来源：

NANO LETTERS | 2011年 / 11卷 / 04期

基金：

美国国家卫生研究院;

关键词：

Integrin; focal adhesion; ECM; micronanostructures; biointerfaces; single cell force spectroscopy (SCFS); ATOMIC-FORCE MICROSCOPY; CYCLIC RGD PEPTIDES; INTEGRIN LIGANDS; MATRIX ADHESIONS; FOCAL ADHESIONS; SURFACES; CELLS;

D O I：

10.1021/nl104079r

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

alpha(v)beta(3) integrin-mediated cell adhesion is crucially influenced by how far ligands are spaced apart. To evaluate the impact of local ligand density versus global ligand density of a given surface, we used synthetic micronanostructured cell environments with user-defined ligand spacing and patterns to investigate cellular adhesion. The development of stable focal adhesions, their number, and size as well as the cellular adhesion strength proved to be influenced by local more than global ligand density.

引用

页码：1469 / 1476

页数：8

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