Production of ethanol from starch by free and immobilized Candida tropicalis in the presence of α-amylase

被引：38

作者：

Jamai, Latifa

Ettayebi, Khalil

El Yamani, Jamal

Ettayebi, Mohamed

机构：

[1] Montana State Univ, Bozeman, MT 59717 USA

[2] SMBA Univ, Biotechnol Units, Atlas, Fez, Morocco

来源：

BIORESOURCE TECHNOLOGY | 2007年 / 98卷 / 14期

关键词：

starch fermentation; candida tropicalis; ethanol production; cell immobilization;

D O I：

10.1016/j.biortech.2006.09.057

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Candida tropicalis is a potentially useful organism for the commercial production of ethanol as it is capable of fermenting starch at a low rate. To enhance this carbon source utilization and increase the rate of alcohol production, we pretreated corn soluble starch with a-amylase. Starch liquefaction was sufficient to drive the fermentation and to convert 96% substrate to ethanol. Indeed, in the presence of exogenous alpha-amylase, 9% (w/v) soluble starch was converted to 43.1 g ethanol/l in 65 h with a productivity of 0.65 g/l h. Thus, bio-ethanol production using free and calcium alginate-immobilized C tropicalis does not require the saccharification step. Furthermore, fed-batch fermentation by free C tropicalis cells increased the final concentration to 56 g ethanol/l, reaching published values for Saccharomyces cerevisiae recombinant strains expressing both alpha-amylase and glucoamylase. (c) 2006 Elsevier Ltd. All rights reserved.

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收藏

页码：2765 / 2770

页数：6

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