The zymogen granule protein 2 (GP2) binds to scavenger receptor expressed on endothelial cells I (SREC-I)

被引：39

作者：

Hoelzl, Markus A. ^{[1
]}

Hofer, Johannes ^{[1
]}

Kovarik, Johannes J. ^{[1
]}

Roggenbuck, Dirk ^{[2
]}

Reinhold, Dirk ^{[3
]}

Goihl, Alexander ^{[3
]}

Gaertner, Miriam ^{[1
]}

Steinberger, Peter ^{[1
]}

Zlabinger, Gerhard J. ^{[1
]}

机构：

[1] Med Univ Vienna, Inst Immunol, Ctr Pathophysiol Infectiol & Immunol, A-1090 Vienna, Austria

[2] GA Gener Assays GmbH, Dahlewitz, Germany

[3] Otto von Guericke Univ, Inst Mol & Clin Immunol, Magdeburg, Germany

来源：

CELLULAR IMMUNOLOGY | 2011年 / 267卷 / 02期

基金：

奥地利科学基金会;

关键词：

SREC-I; GP2; Dendritic cell; THP; Internalization; acLDL; TAMM-HORSFALL GLYCOPROTEIN; MEMBRANE-PROTEIN; RAT PANCREAS; GP-2; RECOGNITION;

D O I：

10.1016/j.cellimm.2010.12.001

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

The pancreatic zymogen granule membrane protein (GP2) is expressed by pancreatic acinar cells and M cells of the ileum. GP2 is the closest related homologue of the urine resident Tamm-Horsfall protein (THP). Recently, it was shown that THP is a ligand of various scavenger receptors (SRs). Therefore, we were interested, if GP2 has similar properties. cDNA of different SRs was stably transfected into a murine thymoma cell line. GP2 was recombinantly expressed, purified and biotinylated. Binding or uptake of GP2 by transfected cells or monocyte-derived dendritic cells (moDCs) was analyzed by flow-cytometry. GP2 is a binding partner of the scavenger receptor expressed on endothelial cells I (SREC-I) but not of SR-AI and SR-BI. The dissociation constant (K-d) of GP2 binding to SREC-I is 41.3 nM. SREC transfected cells are able to internalize GP2. moDCs express SREC-I and also bind and internalize GP2. Inhibition of SREC-I on moDCs with anti-SREC-I antibodies does not result in a decreased GP2 binding. Interaction of GP2 with SREC-I and uptake might have profound effects in antigen clearance and mediation of the immune response. In addition to SREC-I other presently unknown receptors for GP2 on DCs might be involved in this process. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：88 / 93

页数：6

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