Molecular characterization of a novel plasmid-encoded cefotaximase (CTX-M-12) found in clinical Klebsiella pneumoniae isolates from Kenya

被引：67

作者：

Kariuki, S

Corkill, JE

Revathi, G

Musoke, R

Hart, CA

机构：

[1] Kenya Govt Med Res Ctr, Ctr Microbiol Res, Nairobi, Kenya

[2] Kenyatta Natl Hosp, Dept Med Microbiol, Nairobi, Kenya

[3] Liver Unit, Dept Med Microbiol & Genitourinary Med, Liverpool L69 3GA, Merseyside, England

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2001年 / 45卷 / 07期

基金：

英国惠康基金;

关键词：

D O I：

10.1128/AAC.45.7.2141-2143.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Nine Klebsiella pneumoniae isolates, six from blood and three from cerebrospinal fluid of newborn babies at Kenyatta National Hospital, Nairobi, Kenya, were analyzed for the mechanism of cephalosporin resistance. By using pulsed-field gel electrophoresis of XbaI-digested chromosomal DNA, all the nine isolates were found to be clonal. PCR and direct sequencing revealed a novel extended-spectrum beta -lactamase which we designated CTX-M-12. It has a more potent hydrolytic activity against cefotaxime than against ceftazidime and a pI of 9.0 and is encoded on a large self-transferable ca. 160-kbp plasmid.

引用

收藏

页码：2141 / 2143

页数：3

相关论文

共 23 条

[1] A STANDARD NUMBERING SCHEME FOR THE CLASS-A BETA-LACTAMASES [J].

AMBLER, RP ;

COULSON, AFW ;

FRERE, JM ;

GHUYSEN, JM ;

JORIS, B ;

FORSMAN, M ;

LEVESQUE, RC ;

TIRABY, G ;

WALEY, SG .

BIOCHEMICAL JOURNAL, 1991, 276 :269-270

[2] CLOSE AMINO-ACID-SEQUENCE RELATIONSHIP BETWEEN THE NEW PLASMID-MEDIATED EXTENDED-SPECTRUM BETA-LACTAMASE-BULLET-MEN-1 AND CHROMOSOMALLY ENCODED ENZYMES OF KLEBSIELLA-OXYTOCA [J].

BARTHELEMY, M ;

PEDUZZI, J ;

BERNARD, H ;

TANCREDE, C ;

LABIA, R .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1122 (01) :15-22

[3] Sequences of p-lactamase genes encoding CTX-M-1 (MEN-1) and CTX-M-2 and relationship of their amino acid sequences with those of other beta-lactamases [J].

Bauernfeind, A ;

Stemplinger, I ;

Jungwirth, R ;

Ernst, S ;

Casellas, JM .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (02) :509-513

[4] A NOVEL PLASMID-MEDIATED EXTENDED-SPECTRUM BETA-LACTAMASE NOT DERIVED FROM TEM-TYPE OR SHV-TYPE ENZYMES [J].

BERNARD, H ;

TANCREDE, C ;

LIVRELLI, V ;

MORAND, A ;

BARTHELEMY, M ;

LABIA, R .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1992, 29 (05) :590-592

[5] A novel CTX-M β-lactamase (CTX-M-8) in cefotaxime-resistant Enterobacteriaceae isolated in Brazil [J].

Bonnet, R ;

Sampaio, JLM ;

Labia, R ;

De Champs, C ;

Sirot, D ;

Chanal, C ;

Sirot, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (07) :1936-1942

[6] CTX-M-5, a novel cefotaxime-hydrolyzing β-lactamase from an outbreak of Salmonella typhimurium in Latvia [J].

Bradford, PA ;

Yang, YJ ;

Sahm, D ;

Grope, I ;

Gardovska, D ;

Storch, G .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (08) :1980-1984

[7] A FUNCTIONAL CLASSIFICATION SCHEME FOR BETA-LACTAMASES AND ITS CORRELATION WITH MOLECULAR-STRUCTURE [J].

BUSH, K ;

JACOBY, GA ;

MEDEIROS, AA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (06) :1211-1233

[8] CHARACTERIZATION OF A BETA-LACTAMASE PRODUCED BY PSEUDOMONAS-PAUCIMOBILIS [J].

CORKILL, JE ;

HART, CA ;

MCLENNAN, AG ;

ASPINALL, S .

JOURNAL OF GENERAL MICROBIOLOGY, 1991, 137 :1425-1429

[9] Occurrence and detection of AmpC beta-lactamases among Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis isolates at a Veterans Medical Center [J].

Coudron, PE ;

Moland, ES ;

Thomson, KS .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (05) :1791-1796

[10] AMANTADINE PROPHYLAXIS FOR HEALTH-CARE WORKERS - UNANSWERED QUESTIONS [J].

COX, RA .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1991, 27 (01) :1-3