Antibodies from patients with heparin-induced thrombocytopenia stimulate monocytic cells to express tissue factor and secrete interleukin-8

被引:116
作者
Arepally, GM
Mayer, IM
机构
[1] Univ New Mexico, Ctr Hlth Sci, Dept Med, Albuquerque, NM 87131 USA
[2] Univ New Mexico, Ctr Hlth Sci, Canc Res & Treatment Ctr, Albuquerque, NM 87131 USA
关键词
D O I
10.1182/blood.V98.4.1252
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Thrombosis is a life-threatening complication that occurs in a subset of patients with heparin-induced thrombocytopenia (HITT). The pathogenic mechanisms underlying the variable occurrence of thrombosis in HITT is poorly understood. it was hypothesized that monocyte activation leading to tissue factor expression may play a role in promoting a thrombogenic state in HITT. This study demonstrates that a human platelet factor 4 (PF4)/heparin-specific murine monoclonal antibody (KKO) binds to peripheral blood-derived human monocytes in a PF4-dependent manner. KKO and antibodies from patients with HITT induce monocytes to synthesize and secrete interleukin-8 and induce cell-surface procoagulant activity, which is abrogated following treatment with antihuman tissue factor antibody. The findings suggest a novel mechanism by which PF4/heparin antibodies may promote a hypercoagulable, state in patients with HITT.
引用
收藏
页码:1252 / 1254
页数:3
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