Purification and characterization of Moran 20K from Morus alba

被引：43

作者：

Kim, ES ^{[1
]}

Park, SJ ^{[1
]}

Lee, EJ ^{[1
]}

Kim, BK ^{[1
]}

Huh, H ^{[1
]}

Lee, BJ ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Pharm, Seoul 151742, South Korea

来源：

ARCHIVES OF PHARMACAL RESEARCH | 1999年 / 22卷 / 01期

关键词：

Moran; 20K; glycoprotein; MALDI-TOF mass;

D O I：

10.1007/BF02976428

中图分类号：

R914 [药物化学];

学科分类号：

100701 [药物化学];

摘要：

A new glycoprotein was purified from the aqueous methanolic extract of the root bark of Morus alba which has been used as a component of antidiabetic remedy in Oriental Medicine. SDS-PAGE result shows that the molecular weight of the glycoprotein was approximately 20 kDa. This new glycoprotein was named as Moran 20K. The protein lowered blood glucose level in streptozotocin-induced hyperglycemic mice model and it also increased the glucose transport in cultured epididymis fat cells. The amino acid composition of the protein was analyzed, and the protein contained above 20% serine and cysteine such as insulin. The actual molecular weight of the protein was determined as 21,858 Da by MALDI-TOF mass spectroscopy.

引用

页码：9 / 12

页数：4

共 9 条

[1]

IDENTIFICATION AND MAPPING TO CHROMOSOME-1 OF A SUSCEPTIBILITY LOCUS FOR PERIINSULITIS IN NONOBESE DIABETIC MICE [J].