Discrimination of Rhizobium tropici and R-leguminosarum strains by PCR-specific amplification of 16S-23S rDNA spacer region fragments and denaturing gradient gel electrophoresis (DGGE)

被引：19

作者：

de Oliveira, VM

Coutinho, HLC

Sobral, BWS

Guimaraes, CT

van Elsas, JD

Manfio, GP

机构：

[1] Fundacao Andre Tosello, BR-13087010 Campinas, SP, Brazil

[2] Embrapa Solos, Rio De Janeiro, Brazil

[3] Natl Ctr Genome Resources, Santa Fe, NM USA

[4] Res Inst Plant Protect, NL-6700 GW Wageningen, Netherlands

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 28卷 / 02期

关键词：

D O I：

10.1046/j.1365-2672.1999.00480.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

With the aim of detecting Rhizobium species directly in the environment, specific PCR primers for Rh. tropici and Rh. leguminosarum were designed on the basis of sequence analysis of 16S-23S rDNA spacer regions of several lih. tropici, Rh. leguminosarum and Agrobacterium rhizogenes strains. Primer specificity was checked by comparison with available rDNA spacer sequences in databases, and by PCR using DNA from target and reference strains. Sequence polymorphisms of rDNA spacer fragments among strains of the same species were detected by denaturing gradient gel electrophoresis (DGGE). The specific PCR primers designed in this study could be applied to evaluate the diversity of Rh. tropici and Rh. leguminosarum by analysing the polymorphisms of 16S-23S spacer rDNA amplified from either whole-cell or soil-extracted DNA.

引用

收藏

页码：137 / 141

页数：5

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