The effects of AICAR on adipocyte differentiation of 3T3-L1 cells

被引:189
作者
Habinowski, SA
Witters, LA
机构
[1] Dartmouth Coll, Sch Med, Dept Med, Hanover, NH 03755 USA
[2] Dartmouth Coll, Sch Med, Dept Biochem, Hanover, NH 03755 USA
关键词
AMP-activated protein kinase; AICAR (5-aminoimidazole-4-carboxamide; ribonucleoside); 3T3-L1; adipocytes; acetyl-CoA carboxylase; C/EBP (CAAT/enhancer binding protein); PPAR (peroxisome proliferator-activated; receptor);
D O I
10.1006/bbrc.2001.5484
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The AMP-activated protein kinase (AMPK) activator, 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR), has been found to inhibit the differentiation of 3T3-L1 adipocytes, if added at an early phase of differentiation. AICAR blocks the expression of the late adipogenic markers, fatty acid synthase and acetyl-CoA carboxylase, and of the transcription factors, C/EBP alpha and PPAR gamma. It also inhibits early clonal expansion of pre-adipocytes, prevents the fall in C/EBP beta expression during the intermediate stage of differentiation and inhibits the late phase expression of CHOP-10, an antagonist of C/EBP beta. These data suggest a possible inhibitory role for AMPK in the process of adipose differentiation and suggest that AMPK might be a target to block adipogenesis. (C) 2001 Academic Press.
引用
收藏
页码:852 / 856
页数:5
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