Multiple-objective microscopy with three-dimensional resolution near 100 nm and a long working distance

被引：17

作者：

Haeberlé, O ^{[1
]}

Xu, C ^{[1
]}

Dieterlen, A ^{[1
]}

Jacquey, S ^{[1
]}

机构：

[1] Univ Haute Alsace, Inst Univ Technol Mulhouse, Grp Lab El Lab Modelisat Intelligence Proc Syst, F-68093 Mulhouse, France

来源：

OPTICS LETTERS | 2001年 / 26卷 / 21期

关键词：

D O I：

10.1364/OL.26.001684

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The resolution of microscopes is limited by the sizes of their point-spread functions. The invention of confocal, theta, and 4Pi microscopes has permitted the classic Abbe limit to be exceeded. We propose the use of a combination of 4Pi and theta microscopy to decrease resolution by using four illumination objectives and two detection objectives. Using middle numerical aperture, long-working-distance objectives yielded a resolution near 100 mn in the three dimensions, which opens the possibility of exploring large volumes with a high resolution. (C) 2001 Optical Society of America.

引用

页码：1684 / 1686

页数：3

共 15 条

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[2]

CLAPPIER R, 2001, COMMUNICATION

[3] FUNDAMENTAL IMPROVEMENT OF RESOLUTION WITH A 4PI-CONFOCAL FLUORESCENCE MICROSCOPE USING 2-PHOTON EXCITATION [J].