Characterization of phenyl-type HPLC adsorbents

被引：28

作者：

Chan, F

Yeung, LS

LoBrutto, R

Kazakevich, Y

机构：

[1] Seton Hall Univ, Dept Chem & Biochem, S Orange, NJ 07079 USA

[2] Novartis Pharmaceut Corp, PHAD, ARD, E Hanover, NJ 07936 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1069卷 / 02期

关键词：

phenyl-type HPLC adsorbents; LTNA; BET;

D O I：

10.1016/j.chroma.2005.02.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A set of different phenyl-modified HPLC adsorbents were characterized in terms of their surface area, pore volume, and bonded phase volume using low temperature nitrogen adsorption (LTNA). Adsorbents pore volume and interparticle volume were also measured using HPLC. Comparison of the pore volumes assessed with LTNA and HPLC suggests a compact molecular arrangement for all bonded phases studied. Simple and effective method for determination of the exact mass of adsorbent and total surface area in the column is suggested. (c) 2005 Elsevier B.V. All rights reserved.

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收藏

页码：217 / 224

页数：8

相关论文

共 18 条

[1]

*ACD LABS, ACD LABS PHYS CHEM C

[2] NITROGEN ADSORPTION-ISOTHERMS ON ORGANIC AND IONIC MODEL SURFACES [J].

AMATI, D ;

KOVATS, ES .

LANGMUIR, 1987, 3 (05) :687-695

[3] NITROGEN ADSORPTION-ISOTHERMS ON MO2-TYPE OXIDE SURFACES [J].

AMATI, D ;

KOVATS, ES .

LANGMUIR, 1988, 4 (02) :329-337

[4] PREPARATION AND CHROMATOGRAPHIC PROPERTIES OF SOME CHEMICALLY BONDED PHASES FOR REVERSED-PHASE LIQUID-CHROMATOGRAPHY [J].

BERENDSEN, GE ;

GALAN, LD .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1978, 1 (05) :561-586

[5] Adsorption of gases in multimolecular layers [J].

Brunauer, S ;

Emmett, PH ;

Teller, E .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1938, 60 :309-319

[6] THE MOLECULAR MECHANISM OF RETENTION IN REVERSED-PHASE LIQUID-CHROMATOGRAPHY [J].

DORSEY, JG ;

DILL, KA .

CHEMICAL REVIEWS, 1989, 89 (02) :331-346

[7] A NMR TECHNIQUE FOR THE ANALYSIS OF PORE STRUCTURE - APPLICATION TO MATERIALS WITH WELL-DEFINED PORE STRUCTURE [J].

GALLEGOS, DP ;

MUNN, K ;

SMITH, DM ;

STERMER, DL .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1987, 119 (01) :127-140

[8] Retention mechanism in reversed-phase liquid chromatography: Specific effects of propiophenyl and multifunctional (cyanopropyl-octadecyl) bonded silicas [J].

Heron, S ;

Tchapla, A .

JOURNAL OF CHROMATOGRAPHY A, 1996, 725 (02) :205-218

[9] Investigations on the chromatographic behavior of hybrid reversed-phase materials containing electron donor-acceptor systems II.: Contribution of π-π aromatic interactions [J].

Horak, J ;

Maier, NM ;

Lindner, W .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1045 (1-2) :43-58

[10] STUDY OF THE EXCESS ADSORPTION OF THE ELUENT COMPONENTS ON DIFFERENT REVERSED-PHASE ADSORBENTS [J].

KAZAKEVICH, YV ;

MCNAIR, HM .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1995, 33 (06) :321-327