Dendritic patch-clamp recording

被引:105
作者
Davie, Jenny T.
Kole, Maarten H. P.
Letzkus, Johannes J.
Rancz, Ede A.
Spruston, Nelson
Stuart, Greg J.
Haeusser, Michael
机构
[1] UCL, Wolfson Inst Biomed Res, London WC1E 6BT, England
[2] UCL, Dept Physiol, London WC1E 6BT, England
[3] Australian Natl Univ, John Curtin Sch Med Res, Div Neurosci, Canberra, ACT 0200, Australia
[4] Northwestern Univ, Dept Neurobiol & Physiol, Inst Neurosci, Evanston, IL 60208 USA
基金
英国惠康基金;
关键词
D O I
10.1038/nprot.2006.164
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The patch-clamp technique allows investigation of the electrical excitability of neurons and the functional properties and densities of ion channels. Most patch-clamp recordings from neurons have been made from the soma, the largest structure of individual neurons, while their dendrites, which form the majority of the surface area and receive most of the synaptic input, have been relatively neglected. This protocol describes techniques for recording from the dendrites of neurons in brain slices under direct visual control. Although the basic technique is similar to that used for somatic patching, we describe refinements and optimizations of slice quality, microscope optics, setup stability and electrode approach that are required for maximizing the success rate for dendritic recordings. Using this approach, all configurations of the patch-clamp technique (cell-attached, inside-out, whole-cell, outside-out and perforated patch) can be achieved, even for relatively distal dendrites, and simultaneous multiple-electrode dendritic recordings are also possible. The protocol - from the beginning of slice preparation to the end of the first successful recording - can be completed in 3 h.
引用
收藏
页码:1235 / 1247
页数:13
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