Enhanced gene silencing by the application of multiple specific small interfering RNAs

被引：61

作者：

Ji, JM ^{[1
]}

Wernli, M ^{[1
]}

Klimkait, T ^{[1
]}

Erb, P ^{[1
]}

机构：

[1] Univ Basel, Inst Med Microbiol, CH-4003 Basel, Switzerland

来源：

FEBS LETTERS | 2003年 / 552卷 / 2-3期

关键词：

RNA interference; small interfering RNA; gene silencing; CXCR4; fas-ligand; HIV infection;

D O I：

10.1016/S0014-5793(03)00893-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Small interfering RNA duplexes (siRNA) induce gene silencing in various eukaryotic cells, although usually in an incomplete manner. Using chemically synthesized siRNAs targeting the HIV-1 co-receptor CXCR4 or the apoptosis-inducing Fas-ligand (FasL), co-transfection of cells with two or more siRNA duplexes targeting different sites on the same mRNA resulted in an enhanced gene silencing compared with each single siRNA. This was shown in the down-regulation of protein and mRNA expression, and functionally in the inhibition of CXCR4-mediated HIV infection and of FasL-mediated cell apoptosis. Transfection efficiency determined for the FasL-specific siRNAs was dose-dependent and varied among the siRNAs tested, but was not the main reason for the enhanced gene silencing. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：247 / 252

页数：6

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