Lack of mutations in K-ras codons 12 and 13 in human atherosclerotic lesions

被引:5
作者
Bogliolo, M
Fronza, G
Campomenosi, P
Assereto, P
Izzotti, A
Petrilli, GL
Abbondandolo, A
DeFlora, S
机构
[1] NATL INST CANC RES, MUTAGENESIS LAB, CTR STUDY TUMORS ENVIRONM ORIGIN, I-16132 GENOA, ITALY
[2] UNIV GENOA, INST HYG & PREVENT MED, I-16132 GENOA, ITALY
[3] GALLIERA HOSP, DEPT VASC SURG, I-16128 GENOA, ITALY
[4] UNIV GENOA, CHAIR GENET, I-16132 GENOA, ITALY
关键词
atherosclerosis; K-ras; oncogenes;
D O I
10.1016/0009-2797(96)03731-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the framework of a project investigating the possible involvement of cancer biomarkers in human atherogenesis, we evaluated the occurrence of K-ras mutations in the DNA extracted from smooth muscle cells of abdominal aorta atherosclerotic lesions. The molecular analysis of the DNA from 32 surgical specimens, using PCR-based denaturing gradient gel electrophoresis (DGGE), did not reveal any variant in K-ras codons 12 and 13, which are the most frequently involved codons among the ras genes mutated in various types of human tumors. Analysis of the DNA extracted from four cell lines carrying known K-ras mutational alleles showed typically positive DGGE patterns. Thus, on the whole, the conclusions of this study and of previous studies using the same biological material are consistent with the occurrence of DNA adducts in human atherosclerotic lesions but in the absence of p53 involvement or of K-ras mutations in codons 12 and 13. The search for candidate genes which may possibly be involved in the atherogenetic process warrants further studies.
引用
收藏
页码:55 / 62
页数:8
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