pRb2/p130 gene overexpression induces astrocyte differentiation

被引：16

作者：

Galderisi, U

Melone, MAB

Jori, FP

Piegari, E

Di Bernardo, G

Cipollaro, M

Cascino, A

Peluso, G

Claudio, PP

Giordano, A

机构：

[1] Univ Naples 2, Dept Expt Med, Pharmacol Sect, CRISCEB, I-80138 Naples, Italy

[2] Thomas Jefferson Univ, Jefferson Med Coll, Dept Pathol Anat & Cell Biol, Philadelphia, PA 19107 USA

[3] Sbarro Inst Canc Res & Mol Med, Philadelphia, PA USA

[4] Univ Naples 2, Div Neurol 2, Naples, Italy

[5] Univ Naples Federico II, Dipartimento Sci Odontostomatol & Maxillofacciali, Naples, Italy

[6] CNR, Inst Biochem Prot & Enzymol, Arco Felice Napoli, Naples, Italy

来源：

MOLECULAR AND CELLULAR NEUROSCIENCE | 2001年 / 17卷 / 03期

关键词：

D O I：

10.1006/mcne.2000.0949

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

There are many data on the activity of the RE gene in neural differentiation and apoptosis, but the role of pRb2/p130 in neuronal and glial maturation has been far less investigated. To elucidate the role of pRb2/p130 in astrocyte development we overexpressed this protein in astrocytoma and normal astrocyte cultures by adenoviral-mediated gene transfer. In astrocytoma cells, p130/RB2 overexpression resulted in a significant reduction of cell growth and in an increased G(0)/G(1) cell population. We did not observe any induction of programmed cell death as determined by TUNEL reaction. Interestingly, pRb2/p130 overexpression induced astrocyte differentiation. Astrocyte cell cycle arrest and differentiation seemed to proceed through a way distinct from the p53 pathway.

引用

页码：415 / 425

页数：11

共 38 条

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