Cloning of a novel gene yrbB, encoding a protein located in the spore integument of Bacillus subtilis

被引：13

作者：

Takamatsu, H

Hiraoka, T

Kodama, T

Koide, H

Kozuka, S

Tochikubo, K

Watabe, K ^{[1
]}

机构：

[1] Setsunan Univ, Fac Pharmaceut Sci, Osaka 5730101, Japan

[2] Meditopia Res Ctr, Tokyo, Japan

[3] Nagoya City Univ, Sch Med, Dept Microbiol, Nagoya, Aichi 467, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 166卷 / 02期

关键词：

yrbB; Bacillus subtilis; spore integument protein; YrbB;

D O I：

10.1111/j.1574-6968.1998.tb13913.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A DNA fragment (2.7 kbp) containing three deduced open reading frames, orf1, orf2 and orf3 (partial sequence), was isolated from the genomic library of Bacillus subtilis using an antiserum raised against spore integument, and was sequenced. orf2 was 519 nucleotides long and encoded a protein of 172 amino acids with a predicted molecular size of 19 552, corresponding to the protein which reacted with the antiserum. Immunoelectron microscopic observation indicated that YrbB, the product of orf2, was located within the spore integument, mainly in the cortex layer with a part in the inner region of the coat layer. (C) 1998 Published by Elsevier Science B.V. All rights reserved.

引用

页码：361 / 367

页数：7

共 26 条

[1] A BACILLUS-SUBTILIS SPORE COAT POLYPEPTIDE GENE, COTS [J].