Multiple mobile promoter regions for the rare carbapenem resistance gene of Bacteroides fragilis

被引：42

作者：

Podglajen, I

Breuil, J

Rohaut, A

Monsempes, C

Collatz, E

机构：

[1] Univ Paris 06, LRMA, INSERM,E0004, UFR Broussais Hotel Dieu & Pitie Salpetriere, F-75270 Paris 06, France

[2] Ctr Hosp Intercommunal, Bacteriol Lab, Villeneuve St Georges, France

来源：

JOURNAL OF BACTERIOLOGY | 2001年 / 183卷 / 11期

关键词：

D O I：

10.1128/JB.183.11.3531-3535.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two novel insertion sequences (IS), IS1187 and IS1188, are described upstream from the carbapenem resistance gene cfiA in strains of Bacteroides fragilis. Mapping, with the RACE procedure, of transcription start sites of cfiA in these and two other previously reported IS showed that transcription of this rarely encountered gene is initiated close to a variety of B. fragilis consensus promoter sequences, as recently defined (D. P. Bayley, E. R. Rocha, and C. J. Smith, FEMS Microbiol. Lett. 193:149-154, 2000). In the cases of IS1186 and IS1188, these sequences overlap with putative E sigma (70) promoter sequences, while in IS942 and IS1187 such sequences can be observed either upstream or downstream of the B. fragilis promoters.

引用

收藏

页码：3531 / 3535

页数：5

相关论文

共 35 条

[1]

Ausubel F.M., 1992, SHORT PROTOCOLS MOL, V2nd

[2] Analysis of cepA and other Bacteroides fragilis genes reveals a unique promoter structure [J].

Bayley, DP ;

Rocha, ER ;

Smith, CJ .

FEMS MICROBIOLOGY LETTERS, 2000, 193 (01) :149-154

[3] Transcriptional activation of the citrate permease P gene of Lactococcus lactis biovar diacetylactis by an insertion sequence like element present in plasmid pCIT264 [J].

deFelipe, FL ;

Magni, C ;

deMendoza, D ;

Lopez, P .

MOLECULAR AND GENERAL GENETICS, 1996, 250 (04) :428-436

[4] Expression of the carbapenemase gene (cfiA) in Bacteroides fragilis [J].

Edwards, R ;

Read, PN .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2000, 46 (06) :1009-1012

[5] Cloning and sequence analysis of a novel insertion element from plasmids harbored by the carbofuran-degrading bacterium, Sphingomonas sp. CFO6 [J].

Feng, XH ;

Ou, LT ;

Ogram, A .

PLASMID, 1997, 37 (03) :169-179

[6] AN IS1-LIKE ELEMENT IS RESPONSIBLE FOR HIGH-LEVEL SYNTHESIS OF EXTENDED-SPECTRUM BETA-LACTAMASE TEM-6 IN ENTEROBACTERIACEAE [J].

GOUSSARD, S ;

SOUGAKOFF, W ;

MABILAT, C ;

BAUERNFEIND, A ;

COURVALIN, P .

JOURNAL OF GENERAL MICROBIOLOGY, 1991, 137 :2681-2687

[7] Identification of two genetic groups in Bacteroides fragilis by multilocus enzyme electrophoresis:: distribution of antibiotic resistance (cfiA, cepA) and enterotoxin (bft) encoding genes [J].

Gutacker, M ;

Valsangiacomo, C ;

Piffaretti, JC .

MICROBIOLOGY-SGM, 2000, 146 :1241-1254

[8] NUCLEOTIDE-SEQUENCE ANALYSIS OF 2 5-NITROIMIDAZOLE RESISTANCE DETERMINANTS FROM BACTEROIDES STRAINS AND OF A NEW INSERTION-SEQUENCE UPSTREAM OF THE 2 GENES [J].

HAGGOUD, A ;

REYSSET, G ;

AZEDDOUG, H ;

SEBALD, M .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (05) :1047-1051

[9] Modulation of cell surface sialic acid expression in Neisseria meningitidis via a transposable genetic element [J].

Hammerschmidt, S ;

Hilse, R ;

vanPutten, JPM ;

GerardySchahn, R ;

Unkmeir, A ;

Frosch, M .

EMBO JOURNAL, 1996, 15 (01) :192-198

[10]

Hubner A, 1997, J BACTERIOL, V179, P2717

← 1 2 3 4 →