A human CD34(+) subset resides in lymph nodes and differentiates into CD56bright natural killer cells

被引：285

作者：

Freud, AG

Becknell, B

Roychowdhury, S

Mao, HC

Ferketich, AK

Nuovo, GJ

Hughes, TL

Marburger, TB

Sung, J

Baiocchi, RA

Guimond, M

Caligiuri, MA ^{[1
]}

机构：

[1] Ohio State Univ, Integrated Biomed Sci Grad Program, Columbus, OH 43210 USA

[2] Ohio State Univ, Med Sci Program, Columbus, OH 43210 USA

[3] Ohio State Univ, Dept Mol Virol Immunol & Med Genet, Columbus, OH 43210 USA

[4] Ohio State Univ, Dept Epidemiol & Biometr, Columbus, OH 43210 USA

[5] Ohio State Univ, Dept Pathol, Columbus, OH 43210 USA

[6] Ohio State Univ, Div Hematol Oncol, Dept Internal Med, Coll Med & Publ Hlth, Columbus, OH 43210 USA

[7] Ohio State Univ, Comprehens Canc Ctr, Columbus, OH 43210 USA

来源：

IMMUNITY | 2005年 / 22卷 / 03期

关键词：

D O I：

10.1016/j.immuni.2005.01.013

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In humans, T cells differentiate in thymus and B cells develop in bone marrow (BM), but the natural killer (NK) precursor cell(s) and site(s) of NK development are unclear. The CD56(bright) NK subset predominates in lymph nodes (LN) and produces abundant cytokines compared to the cytolytic CD56(dim) NK cell that predominates in blood. Here, we identify a novel CD34(dim) CD45RA(+) hematopoietic precursor cell (HPC) that is integrin alpha(4)beta(bright)(7). CD34(dim)CD45RA(+)beta(bright)(7) HPCs constitute < 1% of BM CD34(+) HPCs and 6% of blood CD34(+) HPCs, but > 95% of LN CD34(+) HPCs. They reside in the parafollicular T cell regions of LN with CD56(bright) NK cells, and when stimulated by IL-15, IL-2, or activated LN T cells, they become CD56(bright) NK cells. The data identify a new NK precursor and support a model of human NK development in which BM-derived CD341(dim)CD45RA(+)beta(bright)(7) HPCs reside in LN where endogenous cytokines drive their differentiation to CD56 (bright) NK cells in vivo.

引用

页码：295 / 304

页数：10

共 43 条

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