Gene silencing by expression of hairpin RNA in Lotus japonicus roots and root nodules

被引:115
作者
Kumagai, H [1 ]
Kouchi, H [1 ]
机构
[1] Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058602, Japan
关键词
D O I
10.1094/MPMI.2003.16.8.663
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We investigated the efficacy of self-complementary hairpin RNA (hpRNA) expression to induce RNA silencing in the roots and nodules of model legume Lotus japonicus, using hairy root transformation mediated by Agrobacterium rhizogenes. Transgenic lines that express P-glucuronidase (GUS) by constitutive or nodule-specific promoters were supertransformed by infection of A. rhizogenes harboring constructs for the expression of hpRNAs with sequences complementary to the GUS coding region. GUS activity in more than 60% of the hairy roots was decreased or silenced almost completely. Silencing of the GUS gene was also observed in symbiotic nodules formed on hairy roots in both early and late stages of nodule organogenesis. These results indicate that transient RNA silencing by hairy root transformation provides a powerful tool for loss-of-function analyses of genes that function in roots and root nodules.
引用
收藏
页码:663 / 668
页数:6
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