Irisin - a myth rather than an exercise-inducible myokine

被引：282

作者：

Albrecht, Elke ^{[1
]}

Norheim, Frode ^{[2
]}

Thiede, Bernd ^{[3
]}

Holen, Torgeir ^{[2
]}

Ohashi, Tomoo ^{[4
]}

Schering, Lisa ^{[1
]}

Lee, Sindre ^{[2
]}

Brenmoehl, Julia ^{[5
]}

Thomas, Selina ^{[6
]}

Drevon, Christian A. ^{[2
]}

Erickson, Harold P. ^{[4
]}

Maak, Steffen ^{[1
]}

机构：

[1] Leibniz Inst Farm Anim Biol, Inst Muscle Biol & Growth, D-18196 Dummerstorf, Germany

[2] Univ Oslo, Inst Basic Med Sci, Dept Nutr, N-0317 Oslo, Norway

[3] Univ Oslo, Biotechnol Ctr Oslo, N-0317 Oslo, Norway

[4] Duke Univ, Dept Cell Biol, Durham, NC 27710 USA

[5] Leibniz Inst Farm Anim Biol, Inst Genome Biol, D-18196 Dummerstorf, Germany

[6] Univ Bern, Vetsuisse Fac, Swiss Inst Equine Med ISME, CH-1580 Avenches, Switzerland

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

关键词：

FNDC5; GENE-EXPRESSION; SKELETAL-MUSCLE; ADIPOSE-TISSUE; CIRCULATING IRISIN; PHYSICAL-ACTIVITY; WHITE FAT; YOUNG; RELEASE; HEALTHY; HUMANS;

D O I：

10.1038/srep08889

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

The myokine irisin is supposed to be cleaved from a transmembrane precursor, FNDC5 (fibronectin type III domain containing 5), and to mediate beneficial effects of exercise on human metabolism. However, evidence for irisin circulating in blood is largely based on commercial ELISA kits which are based on polyclonal antibodies (pAbs) not previously tested for cross-reacting serum proteins. We have analyzed four commercial pAbs by Western blotting, which revealed prominent cross-reactivity with non-specific proteins in human and animal sera. Using recombinant glycosylated and non-glycosylated irisin as positive controls, we found no immune-reactive bands of the expected size in any biological samples. A FNDC5 signature was identified at similar to 20 kDa by mass spectrometry in human serum but was not detected by the commercial pAbs tested. Our results call into question all previous data obtained with commercial ELISA kits for irisin, and provide evidence against a physiological role for irisin in humans and other species.

引用

页数：10

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