Synthesis of modified oligonucleotides and production of duplexes with covalently linked chains

被引：14

作者：

Antsypovich, SI

Oretskaya, TS

Volkov, EM

Romanova, EA

Tashlitsky, VN

Blumenfeld, M

Shabarova, ZA

机构：

[1] MOSCOW MV LOMONOSOV STATE UNIV,DEPT CHEM,MOSCOW 119899,RUSSIA

[2] GENSET,F-75011 PARIS,FRANCE

来源：

NUCLEOSIDES & NUCLEOTIDES | 1996年 / 15卷 / 04期

关键词：

D O I：

10.1080/07328319608002138

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been devised to synthesize a DNA-duplex with covalently connected strands. Primary amino group located on one strand is linked to a carboxyl group of the other strand through the agency of a water soluble carbodiimide condensing agent. Conditions for the reaction between chains of a duplex composed of the modified oligonucleotides [1] were optimized. The thermal and hydrolytic stability of the cross linked duplex was examined.

引用

页码：923 / 936

页数：14

共 12 条

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