Is the photoactive yellow protein a UV-B/blue light photoreceptor?

被引:14
作者
Carroll, Elizabeth C. [1 ]
Hospes, Marijke [2 ]
Valladares, Carmen [1 ]
Hellingwerf, Klaas J. [2 ]
Larsen, Delmar S. [1 ]
机构
[1] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
[2] Univ Amsterdam, Microbiol Lab, SILS, Bioctr, NL-1018 WS Amsterdam, Netherlands
关键词
TRYPTOPHAN FLUORESCENCE; PRIMARY PHOTOREACTION; PHOTOCYCLE; ABSORPTION; ABSORBENCY; PYP;
D O I
10.1039/c0pp00274g
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
UV light below 300 nm is shown to generate the first photocycle intermediate in the blue light photoreceptor Photoactive Yellow Protein. Fluorescence and ultrafast transient absorption measurements indicate two excitation pathways: UV-B absorption by the chromophore and Fluorescence Resonant Energy Transfer (FRET) from tryptophan and tyrosine residues.
引用
收藏
页码:464 / 468
页数:5
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