Scavenging of reactive nitrogen species by mycobacterial truncated hemoglobins

被引：37

作者：

Ascenzi, Paolo ^{[1
,2
,3
]}

Visca, Paolo ^{[1
,2
,3
]}

机构：

[1] IRCCS Lazzaro Spallanzani, Natl Inst Infect Dis, Rome, Italy

[2] Univ Roma Tre, Dept Biol, Rome, Italy

[3] Univ Roma Tre, Interdepartmental Lab Electron Microscopy, Rome, Italy

来源：

GLOBINS AND OTHER NITRIC OXIDE-REACTIVE PROTEINS, PT A | 2008年 / 436卷

关键词：

D O I：

10.1016/S0076-6879(08)36018-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tuberculosis and leprosy are among the most challenging infectious threats to human health. The ability of mycobacteria to persist in vivo in the presence of reactive nitrogen and oxygen species implies the presence in these bacteria of effective detoxification (pseudoenzymatic) systems. Mycobacterium tuberculosis and Mycobacterium leprae truncated hemoglobins (trHbs) belonging to group I (or N; trHbN) and group II (or O; trHbO) have recently been implicated in the scavenging of nitrogen monoxide ((NO)-N-center dot) and peroxynitrite (ONOO-/HOONO). Furthermore, M. leprae trHbO was found to act as an efficient scavenger of the strong oxidant trioxocarbonate((center dot)1(-)) (CO3 center dot-) following the reaction of peroxynitrite with carbon dioxide (CO2). Here, mechanisms for scavenging of reactive nitrogen species by mycobacterial trHbs are reviewed, and detailed protocols for assessing pseudoenzymatic kinetics are provided.

引用

页码：317 / 337

页数：21

共 85 条

[1] Comparison of the roles of reactive oxygen and nitrogen intermediates in the host response to Mycobacterium tuberculosis using transgenic mice [J].