In vitro development of primitive and definitive erythrocytes from different precursors

被引:183
作者
Nakano, T
Kodama, H
Honjo, T
机构
[1] KYOTO UNIV,FAC ENGN,DEPT MED CHEM,SAKYO KU,KYOTO 606,JAPAN
[2] OHU UNIV,SCH DENT,DEPT ANAT,KORIYAMA,FUKUSHIMA 963,JAPAN
关键词
D O I
10.1126/science.272.5262.722
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
During mouse embryogenesis the production of ''primitive'' erythrocytes (EryP) precedes the production of ''definitive'' erythrocytes (EryD) in parallel with the transition of the hematopoietic site from the yolk sac to the fetal liver. On a macrophage colony-stimulating factor-deficient stromal cell line OP9, mouse embryonic stem cells were shown to give rise to EryP and EryD sequentially with a time course similar to that seen in murine ontogeny. Studies of the different growth factor requirements and limiting dilution analysis of precursor frequencies indicate that most EryP and EryD probably developed from different precursors by way of distinct differentiation pathways.
引用
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页码:722 / 724
页数:3
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