Ex vivo expansion and differentiation of unselected peripheral blood progenitor cells in serum-free media

被引：10

作者：

Paquette, RL ^{[1
]}

Gonzalez, E ^{[1
]}

Yoshimura, R ^{[1
]}

Tran, L ^{[1
]}

Choi, R ^{[1
]}

Baldwin, G ^{[1
]}

Slamon, DJ ^{[1
]}

Glaspy, J ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Med, Div Hematol Oncol, Ctr Hlth Sci 42 121, Los Angeles, CA 90095 USA

来源：

JOURNAL OF HEMATOTHERAPY | 1998年 / 7卷 / 06期

关键词：

D O I：

10.1089/scd.1.1998.7.481

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The ability to expand and differentiate unselected PBPC was investigated. Cells were grown in serum-free media containing stem cell factor, GCSF and megakaryocyte growth and development factor (pegylated PEG-rHuMGDF) with or without supplemental serum. Optimal proliferation occurred when PBPC were cultured without prior Ficoll-Paque separation in serum-free media. Cell yields after 17 days of culture were proportional to the percentage of CD34+ cells in the starting population and were 1170 +/- 302-fold higher than the starting numbers of CD34+ cells. Granulocyte-macrophage colony-forming units increased over 12 days of culture, whereas the numbers of erythroid colony-forming cells peaked between 4 and 7 days. Elimination of PEG-rHuMGDF from cell cultures resulted in significantly lower yields of myeloid and erythroid colony-forming cells and total cell numbers. Cell differentiation into neutrophils was indicated by progressive increases in CD11b, CD15, and CD66b expression. Expanded neutrophils phagocytosed and killed bacteria as efficiently as neutrophils from normal donors. Large-scale expansion studies yielded similar proliferation and differentiation results as parallel small-scale cultures. Therefore, unselected PBPC can be efficiently expanded and differentiated into large numbers of functional mature neutrophils.

引用

页码：481 / 491

页数：11

共 27 条

[1] CD34-positive cells isolated from cryopreserved peripheral-blood progenitor cells can be expanded ex vivo and used for transplantation with little or no toxicity [J].