Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy

被引：39

作者：

Takano, T

Miyauchi, A

Yoshida, H

Hasegawa, Y

Kuma, K

Amino, N

机构：

[1] Osaka Univ, Sch Med, Dept Lab Med, Suita, Osaka 5650871, Japan

[2] Kuma Hosp, Chuo Ku, Kobe, Hyogo 6500011, Japan

来源：

BRITISH JOURNAL OF CANCER | 2001年 / 85卷 / 01期

关键词：

thyroglobulin; real-time quantitative RT-PCR; thyroid cancer; molecular-based diagnosis;

D O I：

10.1054/bjoc.2001.1904

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Previous studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of thyroglobulin (TG) mRNA in the peripheral blood of patients with differentiated thyroid carcinoma. To evaluate this usefulness, we measured TG mRNA in the peripheral blood of patients diagnosed with thyroid carcinoma after total thyroidectomy by real-time quantitative RT-PCR using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA as an internal control. Surprisingly, we detected TG mRNA in all samples obtained after total thyroidectomy, including those from 4 medullary carcinomas. Further. there was no statistical difference in expression levels of TG mRNA in the patients with or without metastasis, and no significant correlation was found between serum TG concentrations and the expression levels of TG mRNA. These results give rise to a question regarding the clinical applications of not only RT-PCR detection but also quantitative measurement of TG mRNA in peripheral blood. (C) 2001 Cancer Research Campaign http://www.bjcancer.com.

引用

页码：102 / 106

页数：5

共 15 条

[1]

Bojunga J, 2000, BRIT J CANCER, V82, P1650

[2] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].