Placental glucose transport and GLUT 1 expression in insulin-dependent diabetes

被引:126
作者
Jansson, T
Wennergren, M
Powell, TL
机构
[1] Univ Gothenburg, Dept Physiol & Pharmacol, Perinatal Ctr, S-40530 Gothenburg, Sweden
[2] Univ Gothenburg, Dept Obstet & Gynecol, S-40530 Gothenburg, Sweden
关键词
human pregnancy; glucose transport; placenta;
D O I
10.1016/S0002-9378(99)70169-9
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
OBJECTIVE: Altered transport functions in the placenta might contribute to adverse outcome of pregnancies in women with diabetes. Therefore we studied placental glucose transport in this pregnancy complication. STUDY DESIGN: Syncytiotrophoblast microvillous membrane vesicles and basal membrane Vesicles were isolated from women with uneventful pregnancies (control subjects, n = 21) and from women with pregnancies complicated by insulin-dependent diabetes mellitus, White class D (n = 7). Glucose uptake and GLUT 1 (glucose transporter 1) expression were studied by means of radiolabeled tracers and Western blot, respectively. RESULTS: In the group with insulin-dependent diabetes mellitus, Values for hemoglobin A(1C) were moderately elevated in the first trimester (6.61 +/- 0.35) but not later in pregnancy and 4 of the 7 neonates were large for gestational age. In the basal membrane vesicles, insulin-dependent diabetes mellitus was associated with a 40% increase in GLUT 1 expression and a 59% higher mediated uptake of D-glucose. No alterations could be demonstrated in microvillus membrane vesicles. CONCLUSION: Placental glucose transport capacity appears to be increased in insulin-dependent diabetes mellitus. These alterations might explain the occurrence of macrosomia despite well-controlled diabetes.
引用
收藏
页码:163 / 168
页数:6
相关论文
共 26 条
  • [1] BARTH S, 1994, PLACENTA, V15, pA4
  • [2] OVEREXPRESSION OF GLUT3 PLACENTAL GLUCOSE-TRANSPORTER IN DIABETIC RATS
    BOILEAU, P
    MREJEN, C
    GIRARD, J
    HAUGUELDEMOUZON, S
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1995, 96 (01) : 309 - 317
  • [3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [4] DANDONA P, 1984, LANCET, V1, P737
  • [5] DEVELOPMENTAL EXPRESSION OF GLUT1 GLUCOSE-TRANSPORTER AND C-FOS GENES IN HUMAN PLACENTAL CELLS
    DEMOUZON, SH
    LETURQUE, A
    ALSAT, E
    LOIZEAU, M
    EVAINBRION, D
    GIRARD, J
    [J]. PLACENTA, 1994, 15 (01) : 35 - 46
  • [6] EXPRESSION OF GENES INVOLVED IN PLACENTAL GLUCOSE-UPTAKE AND TRANSPORT IN THE NONOBESE DIABETIC MOUSE PREGNANCY
    DEVASKAR, SU
    DEVASKAR, UP
    SCHROEDER, RE
    DEMELLO, D
    FIEDOREK, FT
    MUECKLER, M
    [J]. AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1994, 171 (05) : 1316 - 1323
  • [7] OF PREGNANCY AND PROGENY
    FREINKEL, N
    [J]. DIABETES, 1980, 29 (12) : 1023 - 1035
  • [8] GAITHER K, 1997, J SOC GYNECOL INVEST, V4, pA103
  • [9] TYPE-I DIABETES-MELLITUS AND PREGNANCY
    GARNER, P
    [J]. LANCET, 1995, 346 (8968): : 157 - 161
  • [10] SIMULTANEOUS PREPARATION OF PAIRED, SYNCYTIAL, MICROVILLOUS AND BASAL MEMBRANES FROM HUMAN PLACENTA
    ILLSLEY, NP
    WANG, ZQ
    GRAY, A
    SELLERS, MC
    JACOBS, MM
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1029 (02) : 218 - 226