Autocrine up-regulation of glucocorticoid receptors by interleukin-6 in human osteoblast-like cells

被引:19
作者
Dovio, A
Masera, RG
Sartori, ML
Racca, S
Angeli, A
机构
[1] Univ Turin, Dipartimento Sci Clin & Biol, Clin Med Gen, I-10043 Orbassano, Italy
[2] Univ Turin, Dipartimento Sci Clin & Biol, Clin Farmacol, Turin, Italy
关键词
osteoblast; interleukin-6; glucocorticoid receptor; glucocorticoid-induced osteoporosis;
D O I
10.1007/s00223-001-2024-8
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Interleukin (IL)-6 is a bone-resorbing cytokine that acts primarily on osteoclast progenitors to stimulate both proliferation and differentiation. Glucocorticoids (GC) down-regulate IL-6 synthesis in different cell types, including osteoblasts. Given the fact that bone remodeling is a tightly controlled process, it is reasonable to think of autoregulatory mechanisms in the bone microenvironment able to prevent excess IL-6 production. We have studied two human osteosarcoma cell lines (Saos-2 and MG-63) with different degrees of differentiation and different constitutive IL-6 production (3.4 +/- 0.2 (mean SE) and 2898 +/- 401 pg/10(6) cells, respectively). We measured the expression of glucocorticoid receptor (GR) in terms of specific binding sites after exposure of cells to different amounts of IL-6. Incubation for 20 hours with IL-6 at increasing concentrations up to 2000 pg/ml yielded significant increase of GR binding sites in both cell lines. IL-6 was also able to revert the inhibitory effect of dexamethasone (1 muM) on GR in both cell lines. In MG-63 cells, that express higher concentrations of GR, IL-6 deprivation via a specific anti-IL-6 antibody (100 ng/ml) significantly decreased GR, as it was noticed, although to a lesser degree, using a specific anti-IL-6 receptor antibody. In Saos-2, cells that express lower concentrations of GR, a 40-hour treatment with human IL-6 1 beta (10 ng/ml) significantly increased both IL-6 production and GR. This latter effect was completely abolished by co-treating the cells with the anti-IL-6 antibody. Our data are consistent with an autocrine up-regulation of GR expression by IL-6 in human osteoblast-like cells. This phenomenon, which is also relevant to paracrine cell-to-cell communication, subserves a feedback loop in the bone microenvironment that restrains excess inducible IL-6 production. In patients having high levels of IL-6 and given GCs, it could offer an additional explanation for the biphasic pattern of bone loss in the course of therapy.
引用
收藏
页码:293 / 298
页数:6
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