Patterns of induced and constitutive monoterpene production in conifer needles in relation to insect herbivory

被引:142
作者
Litvak, ME [1 ]
Monson, RK [1 ]
机构
[1] Univ Colorado, Dept Environm Populat & Organism Biol, Boulder, CO 80309 USA
基金
美国国家航空航天局; 美国国家科学基金会;
关键词
D O I
10.1007/s004420050477
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Studies were conducted to determine whether herbivore-induced synthesis of monoterpenes occurs in the needles of ponderosa pine (Pinus ponderosa Lawson), lodgepole pine (P. contorta Douglas var. latfolia Engelmann), white fir (Abies concolor Lindl. and Gordon) and Engelmann spruce [Picea engelmanii (Parry) Engelm.]. In the needles of all species except Engelmann spruce, simulated herbivory significantly induced the activity of monoterpene cyclases 4-8 days after wounding. In ponderosa pine, real herbivory by last-instar tiger moth larvae (Halisdota ingens Hy. Edwards: Lepidoptera) induced a significantly larger response (4.5-fold increase in monoterpene cyclase activity) than did simulated herbivory (2.5-fold increase). To our knowledge, this is the first report of herbivore-induced increases in monoterpene synthesis in needle tissue. Despite this increase in monoterpene synthesis, we observed no significant increase in total monoterpene pool size in wounded needles compared to controls. Large increases in the rate of monoterpene volatilization were observed in response to wounding. We conclude that the volatile losses caused by tissue damage compensate for herbivore-induced monoterpene synthesis, resulting in no change in pool size. Tiger moth larvae consume ponderosa pine needles in a pattern that begins at the tip and proceeds downward to midway along the needle, at which point they move to an undamaged needle. Constitutive monoterpene concentrations and monoterpene cyclase activities were highest in the lower half of ponderosa pine needles. The monoterpene profile also differed between the upper and lower needle halves, the lower half possessing an additional one to four monoterpene forms. We propose that the increasing gradient in monoterpene concentrations and number of monoterpenes along the needle from tip to base deters feeding beyond the midway point and provides time for the induction of increased cyclase activity and production of new monoterpenes. The induction of new monoterpene synthesis may have a role in replacing monoterpenes lost through damage-induced volatilization and preventing extreme compromise of the constitutive defense system.
引用
收藏
页码:531 / 540
页数:10
相关论文
共 54 条
[1]  
[Anonymous], CHEM MEDIATION COEVO
[2]   THE ALKALOIDAL RESPONSES OF WILD TOBACCO TO REAL AND SIMULATED HERBIVORY [J].
BALDWIN, IT .
OECOLOGIA, 1988, 77 (03) :378-381
[3]   MONOTERPENE AND SESQUITERPENE VARIATION IN PINUS-CARIBAEA NEEDLES AND ITS RELATIONSHIP TO ATTA-LAEVIGATA HERBIVORY [J].
BARNOLA, LF ;
HASEGAWA, M ;
CEDENO, A .
BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 1994, 22 (05) :437-445
[4]  
BERNARD-DAGAN C, 1988, P93
[5]  
BERNARDDAGAN C, 1982, PHYSIOL VEG, V20, P775
[6]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[7]  
BRATTSTEN LB, 1983, ACS SYM SER, V208, P173
[8]  
Bryant J. P., 1988, CHEM MEDIATION COEVO, P367, DOI DOI 10.1016/B978-0-12-656855-4.50016-4
[9]  
Cates R.G., 1990, P169
[10]   RESPONSES OF THE WESTERN SPRUCE BUDWORM TO VARYING LEVELS OF NITROGEN AND TERPENES [J].
CATES, RG ;
HENDERSON, CB ;
REDAK, RA .
OECOLOGIA, 1987, 73 (02) :312-316