Cross-reactivity of an anti-okadaic acid antibody to dinophysistoxin-4 (DTX-4), dinophysistoxin-5 (DTX-5), and an okadaic acid diol ester

被引：9

作者：

Lawrence, JE ^{[1
]}

Cembella, AD

Ross, NW

Wright, JLC

机构：

[1] Dalhousie Univ, Dept Oceanog, Halifax, NS B3H 4J1, Canada

[2] Natl Res Council Canada, Inst Marine Biosci, Halifax, NS B3H 3Z1, Canada

来源：

TOXICON | 1998年 / 36卷 / 08期

关键词：

D O I：

10.1016/S0041-0101(98)00005-1

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The cross-reactivity of the 6/50 monoclonal anti-okadaic acid antibody (mAb) to the recently discovered diarrhetic shellfish poisoning (DSP) metabolites dinophysistoxin-4 (DTX-4), dinophysistoxin-5 (DTX-5), and an okadaic acid (OA) diol ester was determined using a competitive indirect enzyme-linked immunosorbent assay (ELISA). The reactivity of the antibody to these molecules was compared to that with OA; the 6/50 mAb recognized all of these DSP compounds with equal sensitivity within the working range of the antibody (10-100 nM for GA). This confirms the ability of the antibody to detect all DSP compounds when used in analyses including ELISA and immunocytochemistry. Crown copyright (C) 1998 Published by Elsevier Science Ltd. All rights reserved.

引用

页码：1193 / 1196

页数：4

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