We studied the development of soil layers of different pH (strata) within the surface 10 cm of soil. Substrates which affect nitrogen transformations (ammonium sulfate, sucrose, and lucerne hay) were added to the soil to determine the effect of amendment on the development of pH stratification. A glasshouse experiment used soils of 3 different initial pH(0.01 M CaCl2; pH(Ca)) values: 4, 5, and 6. At the end of the experiment, soil was sampled in depth intervals of 0 5 cm between 0 and 2 cm depth and then l-cm intervals between 2 and 10 cm depth. Soil pH(Ca) decreased over time and pH(Ca) changes could be explained by variations in the concentration of ammonium and nitrate, which were brought about by changes in the rate of mineralisation, nitrification, and plant uptake of nitrogen. Little stratification in soil pH(Ca) was found within the 0-10 cm layer. This minimal stratification was considered to be due to the soil in the pots being a closed system where nitrate was not lost through leaching. A field trial was rotary heed to a depth of approximately 10 cm to achieve adequate mixing of soil and amendments. Soil pH(Ca) decreased over the 6 months of the growing season, ranging from 0.1 pH(Ca) units in the control to 0 4 pH(Ca) units in the hay amendment. Between the initial and final sampling times there was little change in soil pH(Ca) in the surface 0-2 cm. The pH(Ca) decreased in the layers between 2 and 10 cm: thus producing the stratification of soil pH(Ca). The growth of oats had little effect on the development of pH(Ca) stratification. Decreases in the soil pH(Ca) from the 0-2 cm to the 8-10 cm layer in the absence of plants were 0.22, 0.27, 0.30, and 0.51 for the control, added sucrose, added ammonium sulfate, and added hay amendments, respectively. The processes of mineralisation and nitrification were considered to be the major causes of change in soil pH(Ca). Stratification of pH(Ca) in the field trial was attributed to nitrification followed by nitrate leaching in the open system.
