The use of cumulative gas and volatile fatty acid production to predict in vitro fermentation kinetics of Italian ryegrass leaf cell walls and contents at various time intervals

被引:34
作者
Groot, JCJ
Williams, BA
Oostdam, AJ
Boer, H
Tamminga, S
机构
[1] Agr Univ Wageningen, Wageningen Inst Anim Sci, Anim Nutr Grp, NL-6709 PG Wageningen, Netherlands
[2] Agr Univ Wageningen, Dept Agron, CT de Wit Grad Sch Prod Ecol, NL-6709 RZ Wageningen, Netherlands
关键词
fermentation; grass; volatile fatty acids;
D O I
10.1079/BJN19980090
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Differences between the fermentation characteristics of cell contents (CC) and protease-treated cell walls (CW) of young leaves of Italian ryegrass (Lolium multiflorum Lam.) cultivar Multimo (tetraploid), were studied in vitro. Gas and volatile fatty acid (VFA) production rates were measured at regular intervals, as was the degradability of organic matter (OM) of CW. The measured VFA were used to predict the gas production and fermentable OM using stoichiometric calculations. For CW the volume and kinetics of measured gas production were the same as those predicted from the VFA formed. In contrast, the measured gas production for CC was consistently less than predicted, indicating that the stoichiometric equations were not valid for rapidly fermenting substrates. For both CC and CW, the relative rate of acetic acid production levelled off more slowly than for other VFA, resulting in an increasing gas yield (in ml/g fermentable OM) after 12 (CW)-24 (CC)h incubation. Consequently, the fermentation of OM was not linearly related to gas production kinetics. For CW, the kinetics of decline of degradable OM and fermentable OM were the same, after correction for a constant 'lost fraction' of degradable OM of 205 g/kg OM. This work indicates the value of detailed studies of fermentation processes to evaluate herbage quality. In particular, the role of CC and the difference between degradation and fermentation require further attention.
引用
收藏
页码:519 / 525
页数:7
相关论文
共 25 条
[1]  
BEUVINK JMW, 1992, APPL MICROBIOL BIOT, V37, P505
[2]   COMPARISON OF INVITRO GAS-PRODUCTION AND NYLON BAG DEGRADABILITY OF ROUGHAGES IN PREDICTING FEED-INTAKE IN CATTLE [J].
BLUMMEL, M ;
ORSKOV, ER .
ANIMAL FEED SCIENCE AND TECHNOLOGY, 1993, 40 (2-3) :109-119
[3]  
CHESSON A, 1993, FORAGE CELL WALL STRUCTURE AND DIGESTIBILITY, P347
[4]  
ERWIN ES, 1959, J ANIM SCI, V18, P1518
[5]  
*GENST 5 COMM, 1993, GENST 5 REL 3 REF MA
[6]  
GOERING HK, 1970, AGR HDB USDA, V279
[7]   Multiphasic analysis of gas production kinetics for in vitro fermentation of ruminant feeds [J].
Groot, JCJ ;
Cone, JW ;
Williams, BA ;
Debersaques, FMA ;
Lantinga, EA .
ANIMAL FEED SCIENCE AND TECHNOLOGY, 1996, 64 (01) :77-89
[8]  
HESPELL RB, 1979, FED PROC, V38, P2707
[9]  
Hungate R.E., 1966, The rumen and its microbes
[10]   POSTPRANDIAL CHANGES IN METHANOGENIC AND ACIDOGENIC BACTERIA IN THE RUMENS OF STEERS FED HIGH-FORAGE OR LOW-FORAGE DIETS ONCE DAILY [J].
LEEDLE, JAZ ;
GREENING, RC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (02) :502-506