Reverse glycoblotting allows rapid-enrichment glycoproteomics of biopharmaceuticals and disease-related biomarkers

被引：48

作者：

Kurogochi, Masaki

Amano, Maho

Fumoto, Masataka

Takimoto, Akio

Kondo, Hirosato

Nishimura, Shin-ichiro

机构：

[1] Hokkaido Univ, Frontier Res Ctr Post Genom Sci & Technol, Grad Sch Adv Life Sci, Div Adv Life Sci, Sapporo, Hokkaido 0010021, Japan

[2] Shionogi & Co Ltd, Osaka 5530002, Japan

[3] Natl Inst Adv Ind Sci & Technol, Drug Seeds Discovery Res Lab, Sapporo, Hokkaido 0628517, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 46期

关键词：

glycoblotting; glycoproteins; proteomics; reverse genomics; sialic acids;

D O I：

10.1002/anie.200702919

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Identification of glycopeptides: A high-throughput glycoproteomics approach was established by using novel reverse-glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N-acetylneuraminic acid; Neu5Gc: N-glycolylneuraminic acid; green: N-acetyl-D-glucosamine; yellow: D-mannose; blue: D-galactose). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：8808 / 8813

页数：6

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