Organic acid excretion by Streptomyces lividans TK24 during growth on defined carbon and nitrogen sources

被引:29
作者
Madden, T
Ward, JM
Ison, AP
机构
[1] UNIV LONDON UNIV COLL,DEPT BIOCHEM & MOL BIOL,LONDON WC1E 6BT,ENGLAND
[2] UNIV LONDON UNIV COLL,DEPT CHEM & BIOCHEM ENGN,ADV CTR BIOCHEM ENGN,LONDON WC1E 7JE,ENGLAND
来源
MICROBIOLOGY-UK | 1996年 / 142卷
关键词
Streptomyces lividans TK24; metabolism; pyruvic acid; 2-oxoglutaric acid; HPLC;
D O I
10.1099/13500872-142-11-3181
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Cultures of Streptomyces lividans TK24 grown in defined media containing certain rapidly used carbon and nitrogen sources excreted high levels of organic acids. These were identified by HPLC and enzymic assays as pyruvic acid and 2-oxoglutaric acid. Acidification occurred only with glucose as the principal carbon source, and depended on the nitrogen source used. With nitrate as the sole nitrogen source. high levels of pyruvate and small amounts of 2-oxoglutarate were produced. Carbon from D-[U-C-14]glucose was converted Biochemistry and into both organic acids. Combining glucose with a selection of amino acids as Molecular Biology, primary nitrogen/secondary carbon sources yielded less pyruvate and more 2-oxoglutarate. Carbon from both C-14-labelled glucose and amino acids was metabolized to both organic acids. Adding nitrate to this combination caused a reversion of the acid production pattern to that of the glucose-nitrate combination. as if the amino acids were absent. Addition of ammonium salts to any combination of carbon and nitrogen sources completely prevented organic acid formation.
引用
收藏
页码:3181 / 3185
页数:5
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