Angiopoietin-2 (Ang-2) is a useful serum tumor marker for liver cancer in the Chinese population

被引：14

作者：

Chen, Yuxin ^{[1
]}

Wu, Yanping ^{[2
]}

Zhang, Xiao ^{[1
]}

Zeng, Hong ^{[3
]}

Liu, Ya ^{[1
]}

Wu, Qi ^{[1
]}

Chen, Yan ^{[1
]}

Zhu, GuoQing ^{[1
]}

Pan, Qiuhui ^{[4
]}

Jin, Lei ^{[5
]}

Guo, Lin ^{[6
,7
]}

Sun, Fenyong ^{[1
]}

机构：

[1] Tongji Univ, Shanghai Peoples Hosp 10, Dept Clin Lab, Middle Yanchang Rd, Shanghai 200072, Peoples R China

[2] Southeast Univ, Zhong Da Hosp, Dept Geriatr, Nanjing 210009, Jiangsu, Peoples R China

[3] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Pathol, Guangzhou 510120, Guangdong, Peoples R China

[4] Shanghai Jiao Tong Univ, Sch Med, Shanghai Childrens Med Ctr, Dept Lab Med, Shanghai 200127, Peoples R China

[5] Shanghai Univ Med & Hlth Sci, Coll Allied Hlth Profess, 279 Zhouzhu Highway, Shanghai 201318, Peoples R China

[6] Fudan Univ, Shanghai Canc Ctr, Dept Clin Lab, Shanghai, Peoples R China

[7] Fudan Univ, Shanghai Med Sch, Dept Oncol, Shanghai 200032, Peoples R China

来源：

CLINICA CHIMICA ACTA | 2018年 / 478卷

基金：

美国国家科学基金会;

关键词：

Liver cancer; Ang-2; Tumor marker; Liver function; Overall survival; HUMAN HEPATOCELLULAR-CARCINOMA; ANGIOGENESIS; EXPRESSION; PROGNOSIS; RECURRENCE; PROTEIN;

D O I：

10.1016/j.cca.2017.12.017

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

100118 [医学信息学]; 100208 [临床检验诊断学];

摘要：

Background: We estimated the diagnostic and prognostic value of serum angiopoietin-2 (Ang-2) in liver cancer patients. Methods: Tissue Ang-2 was measured using immunohistochemistry (IHC). Cell localization of Ang-2 was tested using immunofluorescence (IF). Cell viability and apoptosis were evaluated using MTT and caspase3/7 assays, respectively. Colony-formation was measured using a soft agar assay. Serum Ang-2 was examined using enzyme linked immunosorbent assay (ELISA) and Western blotting. Results: Ang-2 was up-regulated in liver cancer compared to the levels in normal tissues. Serum Ang-2 concentrations were much higher in liver cancer patients than in healthy individuals and those with chronic liver disease (CLD). Inhibitions of Ang-2 using specific shRNA decreased cell proliferation. Serum Ang-2 decreased significantly after surgery. Serum Ang-2 was positively correlated with serum alpha-fetoprotein (AFP; R = 0.375, P = 0.005). Receiver operating characteristic (ROC) curves suggested that serum Ang-2 could be used with relatively high sensitivity and specificity in differentiating liver cancer patients from CLD patients or healthy controls, with corresponding AUC values of 0.742 and 0.924, respectively. Serum Ang-2 was negatively correlated with overall survival. Subgroup analysis also showed that Ang-2 retained its prognostic value in overall survival prediction in different risk subgroups. Conclusion: Serum Ang-2 may be a useful tumor marker in predicting liver cancer prognosis.

引用

页码：18 / 27

页数：10

共 29 条

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Angiopoietin 2 as a therapeutic target in hepatocellular carcinoma treatment: current perspectives [J].