Upregulation of cytosolic phospholipase A2 correlates with apoptosis in mouse superior cervical and dorsal root ganglia neurons

被引：13

作者：

Hornfelt, M ^{[1
]}

Edström, A ^{[1
]}

Ekström, PAR ^{[1
]}

机构：

[1] Univ Lund, Dept Anim Physiol, Lund, Sweden

来源：

NEUROSCIENCE LETTERS | 1999年 / 265卷 / 02期

关键词：

cPLA(2); apoptosis; adult; mouse; superior cervical ganglia; dorsal root ganglia; cell death;

D O I：

10.1016/S0304-3940(99)00046-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The involvement of cytosolic phospholipase A(2) (cPLA(2)) in apoptosis of adult mouse superior cervical and dorsal root ganglia neurons has been investigated by the use of immunohistochemistry for cPLA(2) and DNA nick-end labelling for apoptotic cells, respectively. cPLA(2) immunoreactivity was strongly upregulated in neurons of both preparations during in vitro culturing. By double labeling it was unequivocally demonstrated that cPLA(2) was present and upregulated only in neurons undergoing apoptosis. A similar picture emerged when cPLA(2) immunoreactivity was compared with staining with Fluoro-Jade, a novel fluorochrome marker for neuronal degeneration. The preferential presence of cPLA(2) in apoptotic and degenerating cells suggests that the enzyme is important for some mechanism involved in or intimately coupled to neuronal cell death. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：87 / 90

页数：4

共 21 条

[1]

BONVENTRE JV, 1992, J AM SOC NEPHROL, V3, P128

[2]

BUNT G, 1997, J CELL SCI, V110, P2249

[3] PURIFICATION OF A 110-KILODALTON CYTOSOLIC PHOSPHOLIPASE-A2 FROM THE HUMAN MONOCYTIC CELL-LINE U937 [J].