Hydrophilic interaction liquid chromatography (HILIC) in proteomics

被引：210

作者：

Boersema, Paul J. ^{[1
,2
]}

Mohammed, Shabaz ^{[1
,2
]}

Heck, Albert J. R. ^{[1
,2
]}

机构：

[1] Bijvoet Ctr Biomol Res, Biomol Mass Spectrometry & Proteom Grp, NL-3584 CA Utrecht, Netherlands

[2] Univ Utrecht, Utrecht Inst Pharmaceut Sci, NL-3584 CA Utrecht, Netherlands

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2008年 / 391卷 / 01期

关键词：

HILIC; proteomics; post-translational modification; two-dimensional liquid chromatography;

D O I：

10.1007/s00216-008-1865-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In proteomics, nanoflow multidimensional chromatography is now the gold standard for the separation of complex mixtures of peptides as generated by in-solution digestion of whole-cell lysates. Ideally, the different stationary phases used in multidimensional chromatography should provide orthogonal separation characteristics. For this reason, the combination of strong cation exchange chromatography (SCX) and reversed-phase (RP) chromatography is the most widely used combination for the separation of peptides. Here, we review the potential of hydrophilic interaction liquid chromatography (HILIC) as a separation tool in the multidimensional separation of peptides in proteomics applications. Recent work has revealed that HILIC may provide an excellent alternative to SCX, possessing several advantages in the area of separation power and targeted analysis of protein post-translational modifications.

引用

页码：151 / 159

页数：9

共 83 条

[1] Large-scale identification of N-terminal peptides in the halophilic archaea Halobacterium salinarum and Natronomonas pharaonis
Aivaliotis, Michalis
Gevaert, Kris
Falb, Michaela
Tebbe, Andreas
Konstantinidis, Kosta
Bisle, Birgit
Klein, Christian
Martens, Lennart
Staes, An
Timmerman, Evy
Van Damme, Jozef
Siedler, Frank
Pfeiffer, Friedhelm
Vandekerckhove, Joel
Oesterhelt, Dieter
[J]. JOURNAL OF PROTEOME RESEARCH, 2007, 6 (06) : 2195 - 2204
[2] HYDROPHILIC-INTERACTION CHROMATOGRAPHY FOR THE SEPARATION OF PEPTIDES, NUCLEIC-ACIDS AND OTHER POLAR COMPOUNDS
ALPERT, AJ
[J]. JOURNAL OF CHROMATOGRAPHY, 1990, 499 : 177 - 196
[3] CATION-EXCHANGE CHROMATOGRAPHY OF PEPTIDES ON POLY(2-SULFOETHYL ASPARTAMIDE)-SILICA
ALPERT, AJ
ANDREWS, PC
[J]. JOURNAL OF CHROMATOGRAPHY, 1988, 443 : 85 - 96
[4] ALPERT AJ, 2007, 55 ASMS C MASS SPECT
[5] Electrostatic repulsion hydrophilic interaction chromatography for isocratic separation of charged solutes and selective isolation of phosphopeptides
Alpert, Andrew J.
[J]. ANALYTICAL CHEMISTRY, 2008, 80 (01) : 62 - 76
[6] On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database
Apweiler, R
Hermjakob, H
Sharon, N
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1999, 1473 (01): : 4 - 8
[7] Large-scale characterization of HeLa cell nuclear phosphoproteins
Beausoleil, SA
Jedrychowski, M
Schwartz, D
Elias, JE
Villén, J
Li, JX
Cohn, MA
Cantley, LC
Gygi, SP
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (33) : 12130 - 12135
[8] Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis
Benschop, Joris J.
Mohammed, Shabaz
O'Flaherty, Martina
Heck, Albert J. R.
Slijper, Monique
Menke, Frank L. H.
[J]. MOLECULAR & CELLULAR PROTEOMICS, 2007, 6 (07) : 1198 - 1214
[9] Evaluation and optimization of ZIC-HILIC-RP as an alternative MudPIT strategy
Boersema, Paul J.
Divecha, Nullin
Heck, Albert J. R.
Mohammed, Shabaz
[J]. JOURNAL OF PROTEOME RESEARCH, 2007, 6 (03) : 937 - 946
[10] A high-quality catalog of the Drosophila melanogaster proteome
Brunner, Erich
Ahrens, Christian H.
Mohanty, Sonali
Baetschmann, Hansruedi
Loevenich, Sandra
Potthast, Frank
Deutsch, Eric W.
Panse, Christian
de Lichtenberg, Ulrik
Rinner, Oliver
Lee, Hookeun
Pedrioli, Patrick G. A.
Malmstrom, Johan
Koehler, Katja
Schrimpf, Sabine
Krijgsveld, Jeroen
Kregenow, Floyd
Heck, Albert J. R.
Hafen, Ernst
Schlapbach, Ralph
Aebersold, Ruedi
[J]. NATURE BIOTECHNOLOGY, 2007, 25 (05) : 576 - 583

← 1 2 3 4 5 6 7 8 9 →