A comparison of colorimetric and DNA quantification assays for the assessment of meniscal fibrochondrocyte proliferation in microcarrier culture

被引：21

作者：

Pabbruwe, MB

Stewart, K

Chaudhuri, JB ^{[1
]}

机构：

[1] Univ Bath, Dept Chem Engn, Ctr Regenerat Med, Bath BA2 7AY, Avon, England

[2] Southmead Gen Hosp, Dept Clin Sci N Bristol, Bristol BS10 5NB, Avon, England

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 19期

基金：

英国生物技术与生命科学研究理事会;

关键词：

fibrochondrocytes; meniscal chondrocytes; microcarriers; proliferation;

D O I：

10.1007/s10529-005-1308-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

We have developed and refined a rapid, reliable method for the evaluation of attachment and proliferation of ovine meniscal chondrocytes in microcarrier culture. Assays measuring both mitochondrial activity, using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and MTS [3-(4,5-dimethylthiazol-2-yl)5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium], and DNA synthesis with a PicoGreen assay were compared. The MTT assay was the most sensitive at lower cell concentrations and enabled accurate assessment of cell proliferation over 14 day culture.

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收藏

页码：1451 / 1455

页数：5

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