Capillary electrophoresis in mixed aqueous-organic media:: Effect of tetrahydrofuran on mobilities, dissociation constants and separation of a series of quinolones

被引：15

作者：

Barrón, D ^{[1
]}

Jiménez-Lozano, E ^{[1
]}

Barbosa, J ^{[1
]}

机构：

[1] Univ Barcelona, Dept Analyt Chem, Barcelona 08028, Spain

来源：

CHROMATOGRAPHIA | 2000年 / 52卷 / 7-8期

关键词：

capillary electrophoresis; aqueous-organic media; tetrahydrofuran; quinolones;

D O I：

10.1007/BF02535709

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of the composition of aqueous-twtrahydrofuran mixtures on the electrophoretic behaviour of nine quinolones was studied in order to predict the optimum conditions for their separation. The effect of pH, pK(a) and activity coefficients on electrophoretic behaviour was established. Standard pH values for buffer solutions of the NIST scale as previously determined in THF-water were used in accordance with IUPAC rules. pH was therefore measured for these media as for water. The pK(a) valves of quinolones were determined from the electrophoretic mobilities-pH data pairs in different THF-water mixtures. The electrophoretic resolution between pairs of substances was then predicted in order to obtain the optimal experimental conditions for separation methodologies.

引用

页码：395 / 402

页数：8

共 50 条

[1] Baker D. R., 1995, CAPILLARY ELECTROPHO
[2] Capillary electrophoresis with laser-induced fluorescence detection, an adequate alternative to high-performance liquid chromatography, for the determination of ciprofloxacin and its metabolite desethyleneciprofloxacin in human plasma
Bannefeld, KH
Stass, H
Blaschke, G
[J]. JOURNAL OF CHROMATOGRAPHY B, 1997, 692 (02): : 453 - 459
[3] Preferential solvation in acetonitrile-water mixtures - Relationship between solvatochromic parameters and pK values of carboxylic acids
Barbosa, J
Barron, D
Berges, R
SanzNebot, V
Toro, I
[J]. JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS, 1997, 93 (10): : 1915 - 1920
[4] Dissociation constants and preferential solvation of fluoroquinolones in hydroorganic mixtures used in LC
Barbosa, J
Berges, R
Toro, I
SanzNebot, V
[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1997, 149 (02) : 213 - 225
[5] Factor analysis applied to the correlation between dissociation constants and solvatochromic parameters in water-acetonitrile mixtures .1. Solvent effects on the dissociation of carboxylic acid groups in some diuretics, quinolones, buffers and peptides
Barbosa, J
Fonrodona, G
Marques, I
Buti, S
Toro, I
[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1997, 16 (02) : 104 - 111
[6] Electrophoretic behaviour of quinolones in capillary electrophoresis -: Effect of pH and evaluation of ionization constants
Barbosa, J
Barrón, D
Jiménez-Lozano, E
[J]. JOURNAL OF CHROMATOGRAPHY A, 1999, 839 (1-2) : 183 - 192
[7] Chromatographic behaviour of ionizable compounds in liquid chromatography.: Part 1.: pH scale, pKa and pHs values for standard buffers in tetrahydrofuran-water
Barbosa, J
Barrón, D
Butí, S
[J]. ANALYTICA CHIMICA ACTA, 1999, 389 (1-3) : 31 - 42
[8] Factor analysis applied to the correlation between dissociation constants and solvatochromic parameters in water-acetonitrile mixtures .2. Study of solvent effects on the dissociation of functional groups of diuretics, quinolones, buffers and peptides
Barbosa, J
Marques, I
Fonrodona, G
Barron, D
SanzNebot, V
[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1997, 16 (03) : 140 - 147
[9] Barbosa J, 1999, ELECTROANAL, V11, P627
[10] The application of factor analysis to solvatochromic parameters and pHs values for the standardization of potentiometric sensors in mobile phases used in liquid chromatography
Barbosa, J
Marqués, I
Barrón, D
Sanz-Nebot, V
[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1999, 18 (08) : 543 - 549

← 1 2 3 4 5 →