Characterisation of Salmonella spp. mutants produced by exposure to various fluoroquinolones

被引：19

作者：

Cebrian, L ^{[1
]}

Sirvent, E ^{[1
]}

Díaz, JCR ^{[1
]}

Ruiz, M ^{[1
]}

Royo, G ^{[1
]}

机构：

[1] Univ Miguel Hernandez, Hosp Gen Univ Elche, Microbiol Sect, Alicante 03203, Spain

来源：

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2003年 / 22卷 / 02期

关键词：

Salmonella; fluoroquinolones; mutants;

D O I：

10.1016/S0924-8579(03)00099-2

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We repeatedly exposed six Salmonella spp. strains of different serotypes (three susceptible to nalidixic acid and three resistant) to constant low concentrations of various fluoroquinolones with the aim of characterising the mutations that produce the first decrease in susceptibility to these agents. The fluoroquinolone-susceptibility of all the strains was reduced after repeated exposure to these agents. However, gyr A mutants were not always produced. Furthermore, the type of mutation produced and the time taken for it to appear varied depending on the initial resistance to nalidixic acid, the antibiotic used and the serotype involved. Therefore, we believe that the initial decrease in quinolone-susceptibility is due to various mechanisms and, in many cases, is not caused by mutations in the gyrA gene (C) 2003 Elsevier Science B.V. and the International Society of Chemotherapy. All rights reserved.

引用

页码：134 / 139

页数：6

共 18 条

[1] Mutations of the gyrA gene of clinical isolates of Salmonella typhimurium and three other Salmonella species leading to decreased susceptibilities to 4-quinolone drugs [J].

Brown, JC ;

Thomson, CJ ;

Amyes, SGB .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1996, 37 (02) :351-356

[2]

GENSBERG K, 1995, FEMS MICROBIOL LETT, V132, P57, DOI 10.1016/0378-1097(95)00287-F

[3] Comparative studies of mutations in animal isolates and experimental in vitro- and in vivo-selected mutants of Salmonella spp. suggest a counterselection of highly fluoroquinolone-resistant strains in the field [J].

Giraud, E ;

Brisabois, A ;

Martel, JL ;

Chaslus-Dancla, E .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (09) :2131-2137

[4] Evidence for active efflux as the primary mechanism of resistance to ciprofloxacin in Salmonella enterica serovar typhimurium [J].

Giraud, E ;

Cloeckaert, A ;

Kerboeuf, D ;

Chaslus-Dancla, E .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (05) :1223-1228

[5] Detection of decreased fluoroquinolone susceptibility in salmonellas and validation of nalidixic acid screening test [J].

Hakanen, A ;

Kotilainen, P ;

Jalava, J ;

Siitonen, A ;

Huovinen, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (11) :3572-3577

[6] Identification of DNA gyrase a mutations in ciprofloxacin-resistant isolates of Salmonella typhimurium from men and cattle in Germany [J].

Heisig, P ;

Kratz, B ;

Halle, E ;

Graser, Y ;

Altwegg, M ;

Rabsch, W ;

Faber, JP .

MICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASE, 1995, 1 (03) :211-218

[7] Increasing incidence and comparison of nalidixic acid-resistant Salmonella enterica subsp. enterica serotype Typhimurium isolates from humans and animals [J].

Heurtin-Le Corre, C ;

Donnio, PY ;

Perrin, M ;

Travert, MF ;

Avril, JL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (01) :266-269

[8] Food-related illness and death in the United States [J].

Mead, PS ;

Slutsker, L ;

Dietz, V ;

McCaig, LF ;

Bresee, JS ;

Shapiro, C ;

Griffin, PM ;

Tauxe, RV .

EMERGING INFECTIOUS DISEASES, 1999, 5 (05) :607-625

[9] Mutation rate of avian intestinal coliform bacteria when pressured with fluoroquinolones [J].

Medders, WM ;

Wooley, RE ;

Gibbs, PS ;

Shotts, EB ;

Brown, J .

AVIAN DISEASES, 1998, 42 (01) :146-153

[10] Quinolone resistance mutations in the gyrA gene of clinical isolates of Salmonella [J].

Ouabdesselam, S ;

Tankovic, J ;

Soussy, CJ .

MICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASE, 1996, 2 (03) :299-302

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