Global transcriptional profile of Mycobacterium tuberculosis during THP-1 human macrophage infection

被引：142

作者：

Fontan, Patricia ^{[1
]}

Aris, Virginie ^{[2
]}

Ghanny, Saleena ^{[2
]}

Soteropoulos, Patricia ^{[2
]}

Smith, Issar ^{[1
]}

机构：

[1] Univ Med & Dent New Jersey, New Jersey Med Sch, TB Ctr, Publ Hlth Res Inst, Newark, NJ 07103 USA

[2] Univ Med & Dent New Jersey, New Jersey Med Sch, Ctr Appl Genom, Publ Hlth Res Inst, Newark, NJ 07103 USA

来源：

INFECTION AND IMMUNITY | 2008年 / 76卷 / 02期

关键词：

D O I：

10.1128/IAI.00974-07

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

During lung infection, Mycobacterium tuberculosis resides in macrophages and subverts the bactericidal mechanisms of these professional phagocytes. Comprehension of this host-pathogen relationship is fundamental for the development of new therapies to cure and prevent tuberculosis. In this work, we analyzed the transcriptional profile of M. tuberculosis infecting human macrophage-like THP-1 cells in order to identify putative bacterial pathogenic factors that can be relevant for the intracellular survival of M. tuberculosis. We compared the gene expression profile of M. tuberculosis H37Rv after 4 h and 24 h of infection of human macrophage-like THP-1 cells with the gene expression profile of the strain growing exponentially in broth cultures. We found 585 genes expressed differentially by intracellular M. tuberculosis. An analysis of the gene expression profile of M. tuberculosis inside THP-1 cells suggests the perturbation of the cell envelope as a major intracellular stress inside THP-1 macrophages.

引用

页码：717 / 725

页数：9

共 69 条

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